CircuLex S100A10 ELISA Kit
| Code | Size | Price |
|---|
| MBL-CY-8095 | 96 Assays | £631.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Background:
S100A10, a member of the S100 family of Ca2+-binding proteins, is a dimeric protein composed of
two 11-kDa subunits. The protein is cytosolic when present as a dimer. Typically, S100A10 is found in
most cells bound to annexin A2 as the heterotetrameric (S100A10)2-(annexin A2)2 complex, AIIt, in a
calcium-independent manner (1). The formation of AIIt results in the translocation of S100A10 to the
plasma membrane (2). S100A10 has been shown to regulate plasma membrane ion channels (3, 4) as
well as cytosolic phospholipase A2 (5). In addition to an intracellular distribution, it has also been
established that the heterotetrameric form of S100A10 is present on the extracellular surface of many
cells (6?9). Extracellularly, the S100A10 subunit functions as a plasminogen receptor (10, 11). The
penultimate and ultimate carboxyl-terminal lysines of this subunit bind tPA and plasminogen (12) and
regulate the stimulation of tPA-dependent plasminogen activation (13).
Although S100A10 is expressed fairly ubiquitously in most cells and tissues, high levels of both p11
and A2 were found in 100% of anaplastic thyroid carcinomas, and correlated with their aggressive
behavior (14). In a comprehensive study of S100 gene expression in over 300 primary breast cancers,
both p11 (S100A10) and S100A11 were selectively upregulated in basal versus nonbasal breast cancer
subtypes, but did not predict overall survival (15).
Description:
This kit is used for the quantitative measurement of S100A10 in cell lysate, cell culture supernatant, serum, plasma and other biological media.
Kit Components:
Microplate, 10X Wash Buffer, Dilution Buffer, S100A10 Standard, HRP conjugated Detection Antibody, Substrate Reagent, Stop Solution
Measurement Range:
0.188 ng/mL to 12 ng/mL
Sensitivity:
better than 82.6 pg/ml of sample.
Target:
S100A10
References
1. Donato, R.; S100: a multigenic family of calcium-modulated proteins of the EF-hand type with
intracellular and extracellular functional roles. (2001) Int. J. Biochem. Cell Biol. 33, 637-668
2. Gerke V, Moss SE.; Annexins: from structure to function. (2002) Physiol. Rev. 82, 331-371
3. Girard, C. et al.; p11, an annexin II subunit, an auxiliary protein associated with the background K+
channel, TASK-1. (2002) EMBO J. 21, 4439-4448
4. Okuse, K. et al.; Annexin II light chain regulates sensory neuron-specific sodium channel expression.
(2002) Nature 417, 653-656
5. Wu, T. et al.; P11, a unique member of the S100 family of calcium-binding proteins, interacts with
and inhibits the activity of the 85-kDa cytosolic phospholipase A2. (1997) J. Biol. Chem. 272,
17145-17153
6. Balch, C. and Dedman, J. R.; Annexins II and V inhibit cell migration. (1997) Exp. Cell Res. 237,
259-263
7. Falcone, D. J. et al.; Plasminogen-mediated matrix invasion and degradation by macrophages is
dependent on surface expression of annexin II. (2001) Blood 97, 777-784
8. Kassam, G. et al.; The role of annexin II tetramer in the activation of plasminogen. (1998) J. Biol.
Chem. 273, 4790-4799
9. Mai, J. et al.; Human procathepsin B interacts with the annexin II tetramer on the surface of tumor
cells. (2000) J. Biol. Chem. 275, 12806-12812
10. Kassam, G. et al.; The p11 subunit of the annexin II tetramer plays a key role in the stimulation of
t-PA-dependent plasminogen activation. (1998) Biochemistry 37, 16958-16966
11. Choi, K. S. et al.; p11 regulates extracellular plasmin production and invasiveness of HT1080
fibrosarcoma cells. (2003) FASEB J. 17, 235-246
12. MacLeod, T. J. et al.; Phospholipid-associated annexin A2-S100A10 heterotetramer and its subunits:
characterization of the interaction with tissue plasminogen activator, plasminogen, and plasmin. (2003) J.
Biol. Chem. 278, 25577-25584
13. Fogg, D. K et al.; The p11 subunit of annexin II heterotetramer is regulated by basic
carboxypeptidase. (2002) Biochemistry 41, 4953?4961
14. Ito, K. Arai, R. Nozawa et al.; S100A10 expression in thyroid neoplasms originating from the
follicular epithelium: contribution to the aggressive characteristic of anaplastic carcinoma. (2007)
Anticancer Res. 2679-26783.
15. McKiernan E et al.; The role of S100 genes in breast cancer progression. (2011) Tumour Biol. 32,
441-450