CircuLex Bovine Lactoferrin ELISA Kit
| Code | Size | Price |
|---|
| MBL-CY-8098 | 96 Assays | £602.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Bovine (Cattle)
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Background:
Lactoferrin is an iron-binding glycoprotein of the transferrin family that is expressed in most biologic
fluids and is a major component of mammals' innate immune system (1). It is a glycoprotein consisting
of a single polypeptide chain of about 80 kDa with two globular lobes each containing an iron-binding
site. It is found in most exocrine secretions including milk, particularly the colostrums, and other
secretions, such as tears, saliva, intestinal mucus and genital secretions, and in the specific granules of
neutrophils.
The physiological roles that have been proposed for lactoferrin include anti-inflammatory,
immunomodulatory, antimicrobial, antiviral and antitumoral functions. For this reason, lactoferrin is
regarded as a host-defense mediator. Specific lactoferrin receptors exist in a variety of cells, including
monocytes, lymphocytes, adipocytes, hepatocytes, and endothelial cells (2). Therefore determination of
the lactoferrin concentration in various body fluids can be a marker of inflammation (3-6). It was
reported that fecal lactoferrin is useful as a sensitive and specific marker in identifying intestinal
inflammation such as Crohn?s disease and chronic inflammatory bowel disease (IBD) in human (7).
Combination of several markers, such as S100A8/A9 complex, defensin, elastase, MPO and I-FABP
may be useful for classifying IBD.
Dietary supplements of bovine lactoferrin are purported in consumer literature to enhance and support
the immune system response through their antioxidant, antibacterial, and antiviral properties. Several
scientific researches revealed that oral supplements of bovine lactoferrin may be a useful adjunct toward
modulation of immune activity, in particular T-cell activation and antioxidant status (8).
Description:
The CycLex Research Product CircuLex Bovine Lactoferrin ELISA kit is used for the quantitative
measurement of bovine lactoferrin in biological samples and milk products.
Kit Components:
Microplate, 10xWash Buffer, Dilution Buffer, Bovine Lactoferrin Standard, HRP conjugated Detection Antibody, Substrate Reagent, Stop Solution
Measurement Range:
0.78 ng/mL to 50 ng/mL
Sensitivity:
better than 0.78 ng/mL of sample
Target:
Lactoferrin
References
1. Legrand D, Pierce A, Elass E, Carpentier M, Mariller C, Mazurier J.; Lactoferrin structure and
functions. Adv. Exp. Med. Biol. 606: 163-194, 2008.
2. Ward PP, Paz E, Conneely OM.; Multifunctional roles of lactoferrin: a critical overview. Cell Mol Life
Sci. 62: 2540?8, 2005.
3. Martins CA, Fonteles MG, Barrett LJ, Guerrant RL.; Correlation of lactoferrin with neutrophilic
inflammation in body fluids. Clin Diagn Lab Immunol. 2: 763-5, 1995
4. Rado T A, Bollekens J, St Laurent G, Parker L, Benz E J Jr.; Lactoferrin biosynthesis during
granulocytopoiesis. Blood 64: 1103-9, 1984
5. Guerrant RL, Araujo V, Soares E, Kotloff K, Lima AA, Cooper WH, Lee AG.; Measurement of fecal
lactoferrin as a marker of fecal leukocytes. J Clin Microbiol. 30: 1238-42, 1992
6. Parsi MA, Saadeh SN, Zein NN, Davis GL, Lopez R, Boone J, et al.; Ascitic fluid lactoferrin for
diagnosis of spontaneous bacterial peritonitis. Gastroenterology 135: 803-7, 2008
7. Buderus S, Boone J, Lyerly D, Lentze MJ.; Fecal lactoferrin: a new parameter to monitor infliximab
therapy. Dig Dis Sci. 49: 1036-9, 2004
8. Mulder AM, Connellan PA, Oliver CJ, Morris CA, Stevenson LM.; Bovine lactoferrin
supplementation supports immune and antioxidant status in healthy human males.
Nutr Res. 28: 583-9, 2008