CircuLex Human DJ-1/PARK7 ELISA Kit Ver.2
| Code | Size | Price |
|---|
| MBL-CY-9050V2 | 96 Assays | £602.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Background:
DJ-1 (PARK7/CAP1/RS) was originally cloned as a putative oncogene capable of transforming
NIH-3T3 cells in cooperation with H-ras (1), a protein expressed in sperm (2), and a regulator of
RNA-protein interactions (3). DJ-1 has also been isolated as a gene associated with autosomal
early-onset Parkinson's disease (PD) (4). Taken together, DJ-1 appears to be involved in diverse
biological processes (5). First, several lines of evidence suggest that DJ-1 plays a role in the oxidative
stress response (6,7). In cu1tured mammalian ce11s, DJ-1 quenches reactive oxygen species and is
converted into a variant with a more acidic isoelectric point (6,8). Therefore, DJ-1 protects against
reactive oxygen species-induced cell death, and its suppression with small interfering RNA (siRNA)
sensitizes cells to such insults (7-10). Second, DJ-1 modulates transcription through interaction with
DJ-1-binding protein (11) as well as with protein inhibitor of activated STAT (PIAS) (12). The latter
modulates the activity of various transcription factors, Third, DJ-1 has been recognized as a regulatory
subunit of an RNA-binding protein (13), Fourth, DJ-1, which is structurally related to the molecular
chaperone Hsp31, may have chaperone activity itself, preventing heat-induced aggregation of substrate
proteins, including alpha-synuclein (14).
In addition, severa1 1ines of evidence suggest that DJ-1 plays a role in human tumorigenesis. First,
breast cancer patients have elevated levels of circulating DJ-1 and anti-DJ-1 autoantibodies compared to
healthy and non-breast cancer patients (15). Secondly, DJ-1 protein is increased in primary non-small
cell lung carcinoma samples (16). Thirdly, treatment of cells from the human lung cancer cell line
NCI-H157 with paclitaxel and MEK inhibitor U0126 leads to a decrease in DJ-1 protein expression (16).
Description:
The CycLex Research Product CircuLex Human DJ-1/PARK7 ELISA kit Ver.2 is used for the
quantitative and highly sensitive measurement of human DJ-1/PARK7 in serum, plasma, cell lysate and
other biological samples.
Kit Components:
Microplate, 10X Wash Buffer, Dilution Buffer, Human DJ-1/PARK7 Standard, 20X HRP conjugated Detection Antibody, Conjugate Dilution Buffer, Substrate Reagent, Stop Solution
Measurement Range:
25 pg/mL to 1,600 pg/mL
Sensitivity:
better than 8.4 pg/mL of sample.
Target:
DJ-1/PARK7
References
1. D. Nagakubo, T. Taira, H. Kitaura, M. lkeda, K. Tamai, S.M. Iguchi-Ariga and H. Ariga: Biochem.
Biophys.Res.Commun.231, 509-513, 1997
2. A. Wagenfeld, J. Gromoll and T.G, Cooper Biochem. Biophys. Res. Commun. 251, 545-549, 1998
3. Y. Hod, S.N. Pentyala, T.C. Whyard and M.R. El-Maghrabi: J. Cell. Biochem. 72, 435-444, 1999
4. Bonifati, V., Rizzu, P., van Baren, M. J., Schaap, O., Breedveld, G. J., Krieger, E., Dekker, M. C. J,
Squitieri, F., Ibanez, P., Joosse, M., van Dongen, J.W., Vanacore, N., van Swieten, J.C, Brice, A.,
Meco, G., van Duijng, C. M., Oostra, B. A., Heutink, P.: Science 299, 256-259, 2003.
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Baptista, M. J., Ringe, D., Petsko, G. A. & Cookson, M. R.: Proc. Natl. Acad. Sci. USA 101,
9103-9108, 2004
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2004
9. Martinat, C., Shendelrnan, S., Jonason, A., Leete, T., Bea1, M,F., Yang, L., Floss, T. & Abeliovich,
A.: PLoS Biol, 2, e327, 2004
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Commun, 312, 1342-1348, 2003
11. Niki, T., Takahashi-Niki, K., Taira, T., Iguchi-Ariga, S. M. & Ariga, H.: Mol. Cancer Res. 1,
247-261, 2003
,
12. Takahashi, K., Taira, T., Niki, T., Seino, C., lguchi-Ariga, S. M. & Ariga, H.: J. Biol. Chern. 276,
37556-37563, 2001
13. Hod, Y., Pentyala, S.N., Whyard, T.C.& El-Maghrabi, M.R.: J. Cell Biochem.72, 435-444, 1999
14. Shendelman, S., Jcmason, A., Manimag, C., Leete, T. & Abeliovich, A.: PLoS Biol.2, e362, 2004
15. F. Le Naour, D.E. Misek, M. C. Krause, L. Deneux, T. J. Giordano, S. Scholl and S.M. Hanash: Clin.
Cancer Res. 7, 3328-3335, 2001