Akt1 Positive Control

Product Code: MBL-CY-E1168-1

Supplier: MBL

Code	Size	Price

MBL-CY-E1168-1

200 Assays

£518.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO

Shipping:

Dry Ice

Storage:

-70°C

Documents

Datasheet

Further Information

Applications:

Other - 25 milliunits/well

Background:

Active human AKT1 containing an N-terminal GST tag and a C-terminal His tag was produced by co-infection of PDK-1 and full length AKT1-expressing recombinant baculovirus into Sf9 cells. Purified by GSH agarose chromatography

Formulation:

Supplied frozen in 20 mM HEPES-KOH (pH 7.5), 1%BSA, 1 mM EDTA, 1 mM DTT, 50 mM NaCl, 0.03%Brij35 and 50% glycerol.

Gene IDs:

Human: 207 Mouse: 11651

MW:

Recombinant Akt1 demonstrates a doublet at 92 kDa by SDS-PAGE analysis.

Purity:

>85% pure as determined by SDS-PAGE analysis.

Shelf Life:

1 year

Target:

AKT1

References

1. Staal, S.P. Proc. Nat. Acad. Sci. 84, 5034-5037, 1987. 2. Andjelkovic M, et al. J Biol Chem. 272, 31515-24, 1997 3. Meier, R., et al. J. Biol. Chem. 272, 30491?30497, 1997 4. Cross, D. et al. Nature 378, 785-789, 1995 5. Kohn, A.D., et al. J. Biol. Chem. 271, 31372-31378, 1996 6. Dudek, H., et al. Science 275, 661-665, 1997 7. Cheng, J.Q., et al. Proc. Nat. Acad. Sci. 89, 9267-9271, 1992 8. Bellacosa, A. et al. Int. J. Cancer 64, 280-285, 1995 9. Cheng, J.Q., et al. Proc. Nat. Acad. Sci. 93, 3636-3641, 1996 10. Vanhaesebroeck, B.; Alessi, D.R. Biochem. J. 346, 561- 576, 2000. (Review) 11. Franke, T. F., et al. Cell 88, 435-437, 1997 (Review) 12. Hemmings, B.A. Science 275, 628-630, 1997 (Review).