CK2 (alpha/beta) Positive Control

Other - 20 milliunits/well.

Protein kinase CK2 is a ubiquitous and pleiotropic serine/threonine protein kinase that appears to interact with many different signaling pathways. The holoenzyme is generally composed of two catalytic (alpha and/or alpha') and two regulatory (beta) subunits, but the free alpha/alpha' subunits are catalytically active by themselves. Although the beta subunits deeply affect many properties of CK2, both the isolated catalytic subunits and the holoenzyme are constitutively active. The enzyme is highly expressed in most cancers and this higher expression has been tentatively correlated with the involvement of CK2 in the promotion of specific phases of the cell cycle. It is highly conserved and is known to phosphorylate about 300 different proteins. Unlike the majority of protein kinases, which are tightly regulated enzymes, CK2 is endowed with high constitutive activity, a feature that is suspected to underlie its oncogenic potential and possible implication in viral infections. This makes CK2 an attractive target for anti-neoplastic and antiviral drugs.

Supplied frozen in a buffer containing 20mM HEPESKOH(pH 7.5), 1% BSA, 1 mM DTT, 50mM NaCl, 0.03% Brij35 and 50% glycerol.

Human: 1457 Mouse: 12995

CK2 (alpha/beta) demonstrates 43 kDa and 30 kDa bands respectively by SDS-PAGE analysis.

>65% pure as determined by SDS-PAGE analysis.

1 year