CaM-kinase II Positive Control

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MBL
Product Code: MBL-CY-E1173
Product Group: Recombinant Proteins
Supplier: MBL
CodeSizePrice
MBL-CY-E1173200 Assays£430.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Shipping:
Dry Ice
Storage:
-70°C

Documents

Further Information

Applications:
Other - 15 milliunits/well
Background:
Active human CaM kinase II, residues 1-328, containing an N-terminal GST tag and C-terminal His tag was produced by infection of C-terminal truncated CaM kinase IIexpressing recombinant baculovirus into Sf9 cells. Purified by GSH-Sepharose and Ni-NTA agarose chromatography
Formulation:
Supplied frozen in 20 mM HEPES-KOH (pH 7.5), 1%BSA, 1 mM EDTA, 1 mM DTT, 50 mM NaCl, 0.03%Brij35 and 50% glycerol.
Gene IDs:
Human: 816 Mouse: 12323
MW:
Recombinant CaM Kinase II demonstrates a doublet at 62 kDa by SDS-PAGE analysis.
Purity:
>90% pure as determined by SDS-PAGE analysis.
Shelf Life:
1 year
Target:
CaM II

References

1. Hudmon, A., and Schulman, H. Biochem. J. 364, 593- 611, 2002. 2. Van Eldik, L., and Watterson, M. Calmodulin and Signal Transduction, Academic Press, New York, 1998 3. Erondu, N. E. and Kennedy, M. B. J. Neurosci. 5, 3270-3277, 1985 4. Soderling, T. R. Biochim. Biophys. Acta 1297, 131? 138, 1996 5 Kolodziej, S. J., Hudmon, A., Waxham, M. N., and Stoops, J. K. J. Biol. Chem. 275, 14354?14359, 2000 6. Kanaseki, T., Ikeuchi, Y., Sugiura, H., and Yamauchi, T. J. Cell Biol. 115, 1049-1060, 1991 7. Brocke, L., Chiang, L. W., Wagner, P. D., and Schulman, H. J. Biol. Chem. 274, 22713?22722, 1999