Anti-Phospho-Lats2 (Ser83) mAb

WB - 1-2 ug/mL ELISA - 1 ug/mL

Lats2 is a new member of the Lats tumor suppressor family. The human LATS2 gene is located at chromosome 13q11-12, which has been shown to be a hot spot (67%) for LOH in nonsmall cell lung cancer. The Drosophila ?large tumor suppressor? (lats) gene encodes a putative serine/threonine kinase. By screening a human testis cDNA library with a mouse LATS2 cDNA, Yabuta et al. isolated a partial human LATS2 cDNA encoding a deduced 1,046-amino acid C-terminal partial protein. LATS2 contains a PAPA repeat consisting of 7 copies of the dipeptide proline-alanine, which may be involved in protein-protein interactions, and a C-terminal Ser/Thr kinase domain. Analysis of the amino acid sequences of the human and mouse LATS2 kinase domains indicated that the LATS2 proteins are most closely related to the LATS1 serine/threonine kinases, followed by Drosophila Lats. Immunoblot analysis of human cell lines showed that endogenous LATS2 is a nuclear protein of approximately 125 kDa. Northern blot analysis detected a 5.8-kb LATS2 transcript in several human tissues, with highest expression in heart and skeletal muscle.

1.0 mg/mL

Supplied in 20mM phosphatase buffer (pH 7.5), 300mM NaCl, 50% glycerol.

Human: 26524 Mouse: 50523

Near the Ser83 phosphorylated peptide synthesis of human Lats2 (binding KLH)

Recognizes phosphorylated Lats2 in human cell lysates. Not tested in other species.

1 year

Monoclonal antibody was produced by immunizing mice with a synthetic phosphopeptide corresponding to residues surrounding Ser83 of human Lats2. IgG is purified by protein A-Sepharose chromatography.

Lats2