Anti-Phospho-Syntide-2 mAb

ELISA - 1 ug/mL

Syntide-2, a peptide based on phosphorylation site two of glycogen synthase, is an exogenous substrate designed for CaM-Kinase (calcium/calmodulin dependent kinases) in the serine-threonine kinase family. CaM-kinase II is a multifunctional calcium/calmodulin dependent protein kinase involved in neuronal functions. This anti-Phospho-Syntide-2 monoclonal antibody has been validated with CaM-kinase II, however it has the potential for use in evaluating other serine/threonine kinases such as CaM-Kinase IIa, PKCu, Akt1, Akt2, Akt3, and PKA. The relative Vmax/Km ratios of the known Ca2+-dependent protein kinases for syntide-2 were determined to be as follows: protein kinase II, 100; protein kinase C, 22; phosphorylase kinase, 2; myosin light chain kinase, 0.005.

1.0 mg/mL

Supplied in 20mM phosphatase buffer (pH 7.5), 300mM NaCl, 50% glycerol.

a synthetic phosphopeptide Syntide-2 (PLARTL(pS)VAGLPGKK; synthetic substrate for CaM-Kinase II)

Phospho-Syntide-2 Antibody detects phosphorylated Syntide-2 only when phosphorylated at the serine residue in vitro. The antibody does not recognize the unphosphorylated substrate. The antibody detects the phosphorylated Syntide-2 by ELISA.

1 year

This monoclonal antibody (clone MS-6E6) was produced by immunizing mice with a synthetic phosphopeptide Syntide-2, PLARTL(pS)VAGLPGKK, which is synthetic substrate for CaM-Kinase II (Calmodulin dependent protein kinase II). The IgG fraction was purified by protein A-Sepharose chromatography.

Syntide-2