Anti-Baculovirus Envelope gp64 mAb
| Code | Size | Price |
|---|
| MBL-CY-M1026 | 50 ug | £301.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: AF-2C8
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Flow Cytometry
- Immunocytochemistry (ICC)
- Immunofluorescence (IF)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
IF - 0.5-1 ug/mL
Background:
Recombinant baculoviruses derived from the Autographa californica nuclear polyhedrosis virus (AcNPV) are widely used to express heterologous genes in insect cells, but the use of the baculovirus expression vector system is hampered by slow and tedious procedures for the selection and propagation of baculovirus and for titer determination. Titration of baculoviral stocks is important because it is critical to have consistency between samples, and to achieve the right level of transient expression. Moreover, it is important to know the titer of infectious particles when producing viral stocks for successful virus production. This antibody can be used for determining titers of baculovirus stocks by immunocytochemical or immunofluorescence methods.
Concentration:
1.0 mg/mL
Formulation:
Supplied in 20mM phosphatase buffer (pH 7.5), 300mM NaCl, 50% glycerol.
Immunogen Translated:
Wild-type AcNPV particles
Reactivity:
Baculovirus envelope gp64 antibody detects the gp64 envelope protein of
baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV).
Shelf Life:
1 year
Source:
Monoclonal antibody is produced by immunizing mice with wild type AcNPV
particles. IgG is purified by protein A-sepharose chromatography.
Target:
Baculovirus Envelope gp64
References
(1) Hohmann, A. W. and P. Faulkner. 1983. Monoclonal antibodies to baculovirus structural proteins:
determination of specificities by Western blot analysis. Virology 125(2): 432-44.
(2) Volkman, L. E. and P. A. Goldsmith. 1988. Resistance of the 64K protein of budded Autographa
californica nuclear polyhedrosis virus to functional inactivation by proteolysis. Virology 166(1):
285-9.
(3) Blissard, G. W. and G. F. Rohrmann 1989. Location, sequence, transcriptional mapping, and
temporal expression of the gp64 envelope glycoprotein gene of the Orgyia pseudotsugata
multicapsid nuclear polyhedrosis virus. Virology 170(2): 537-55.
(4) Plonsky, I., M. S. Cho, et al. (1999). An analysis of the role of the target membrane on the
Gp64-induced fusion pore. Virology 253(1): 65-76.