CircuLex Glyceraldehyde-AGE-BSA
| Code | Size | Price |
|---|
| MBL-CY-R2058 | 200 ug | £285.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
dry ice
Storage:
-20°C
Documents
Further Information
Background:
Reducing sugars react with protein amino groups to form a diverse
group of protein-bound moieties with fluorescent and cross-linking
properties. These compounds, called advanced glycosylation end
products (AGEs), have been implicated in the structural and functional
alterations of proteins that occur during aging and long-term diabetes.
Although several AGE structures have been reported (1, 2),
Nε-(Carboxymethyl) lysine (CML) and Nε-(carboxyethyl)lysine (CEL)
are two stable, nonenzymatic chemical modifications of protein lysine
residues resulting from glycation and oxidation reactions. However it
has been proposed that AGE are not only created from glucose per se,
but also from dicarbonyl compounds derived from glycation, sugar
autoxidation, and sugar metabolism. Various types of AGE,
non-enzymatically glycated protein derivatives formed at an
accelerated rate in diabetes (3), have been demonstrated to inhibit DNA
synthesis and also induce apoptotic cell death in human mesangial
cells (4).
Concentration:
1.0 mg/mL
Formulation:
The glyceraldehyde-AGE-BSA is supplied frozen in a buffer containing
10 mM PBS (pH 7.2).
Shelf Life:
1 year
Target:
Glyceraldehyde-AGE-BSA
References
(1) Ikeda K, Higashi T, Sano H, Jinnouchi Y, Yoshida M, Araki T,
Ueda S, Horiuchi S: Biochemistry 35: 8075 ?8083,1996
(2) Reddy S, Bichler J, Wells-Knecht KJ, Thorpe SR, Baynes JW:
Biochemistry 34: 10872?10878, 1995
(3). Takeuchi, M., Bucala, R., Suzuki, T., Ohkubo, T., Yamazaki,
M., Koike, T., Kameda, Y., and Makita, Z. (2000) J. Neuropathol.
Exp. Neurol. 59: 1094-1105
(4). Yamagishi S, Inagaki Y, Okamoto T, Amano S, Koga K,
Takeuchi M, Makita Z. J. Biol. Chem. 277:20309-15, 2002
(5) Takeuchi M, Makita Z, Bucala R, Suzuki T, Koike T, Kameda Y
Mol Med. 6: 14-25, 2000
(6) R Nagai, K Matsumoto, X Ling, H Suzuki, T Araki, and S
Horiuchi: Diabetes, 49: 1714 ? 1723, 2000