Anti-KOR-SA3544 (Human) mAb-FITC
Code | Size | Price |
---|
MBL-D028-4 | 50 Tests | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: KOR-SA3544
Regulatory Status: RUO
Target Species: Human
Applications:
- Flow Cytometry
- Western Blot (WB)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
WB - 10 ug/mL FCM - 20 uL
Background:
This monoclonal antibody (clone KOR-SA3544) was reactive with a surface antigen expressed on Philadelphia chromosome (Ph1)-positive acute lymphoblastic leukemia (ALL) without exception (26/26 cases). The recognized antigen is a nonspecific cross-reacting antigen (NCA)-50/90 (CD66c), one of the carcinoembryonic antigen (CEA)-related glycoproteins encoded by a member of the CEA gene family. The Philadelphia chromosome (Ph1) has been implicated as the causative factor in greater than 90% of chronic myelogenous leukemia (CML), in 25-30% of adult and 2-10% of childhood acute lymphoblastic leukemia (ALL) and in rare cases of acute myelogenous leukemia (AML). The presence of the Ph in leukemic cells of ALL patients usually indicates poor prognosis and high risk. Sequential monitoring of the Ph in ALL correlates with the activity of malignant clones and predicts impending clinical relapse, and therefore is useful in guiding clinical therapeutic decisions.
Conjugate:
FITC
Formulation:
20 ug/mL in PBS/1% BSA/0.09% sodium azide
Gene IDs:
Human: 4680
Immunogen Translated:
KOCL-22 myeloid cell line derived from congenital leukemia
Reactivity:
The reactivity of this antibody has been
reported as follows.
Common ALL
a
5/38
(13.2%)
Early B precursor ALL
b
1
c
/21
(4.8%)
T
-
ALL
0/19
B
-
ALL
0/6
B
-
CLL/HCL
0/3
Multiple myeloma
0/2
ANLL
16/56
(28.6%)
Ph1
-
ALL
26/26
d
(100%)
CML blastic crisis
0/9
T
-
NHL
0/5
B
-
NHL
0/4
Hodgkin's disease
0/1
CLL, chronic lymphocytic leukemia
HCL, hairy cell leukemia
NHL, non
-
Hodgkin's lymphoma
a
C
D10
+
,
CD19
+
,
HLA
-
DR
+
b
CD10
-
,
CD19
+
,
HLA
-
DR
+
c
One patient with 11q23 translocation.
d
Eighteen patients with m
-
bcr, eight patients with M
-
bcr
Shelf Life:
1 year
Source:
This antibody was
purified
from
ascites fluid
(clone KOR
-
SA3544) using
protein A
agarose. This
hybr
idoma
was established by fusion of mouse myeloma
cell 63.Ag8.653/NS1
with Balb/c mouse splenocyte
immunized with
a
cell line (KOCL
-
22
)
established from
bone marrow blood of patient with congenital
leukemia.
Target:
KOR-SA3544
References
1)
Sugita, K.,
et al.,
Leukemia
13
,
779
-
785
(199
9
)
2)
Mori
,
T.,
et al.,
Leukemia
9
, 1233
-
1239 (1995)
Clone
KOR
-
SA3544 is used in these references.