Anti-Proton Pump (H,K-ATPase beta subunit) (Mouse) mAb
Code | Size | Price |
---|
MBL-D032-3 | 100 ug | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 2B6
Regulatory Status: RUO
Target Species:
- Bovine (Cattle)
- Human
- Mouse
- Rat
Applications:
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
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Further Information
Applications:
WB - 2-10 ug/mL (chemiluminescence detection system) IP - 5-10 ug/200-300 uL of cell extract IHC - 2-10 ug/mL
Background:
Hydrogen-potassium adenosine
triphosphatase (H
+
, K
+
-ATPase) belongs to a family of
P-type cation-transporting ATPases that also includes
Ca
2+
-ATPase and Na
+
, K
+
-ATPase. H
+
, K
+
-ATPase is
responsible for acid secre
tion into the stomach and
catalyzes electroneutral excha
nge of cytoplasmic hydrogen
ions and external potassium ions coupled with ATP
hydrolysis. The enzyme is found in gastric parietal cells
where it is located in cytoplasmi
c vesicles or apical plasma
membranes of the secretory canaliculus. H
+
, K
+
-ATPase is
a heterodimer consisting of a high molecular weight
catalytic
α
subunit and a smaller but heavily glycosylated
α
subunit. The
α
subunit of H
+
, K
+
-ATPase has 10
transmembrane domains and
contains sites for ATP
binding and its acylphosphorylation, binding sites of
proton pump inhibitors, and sites responsible for ion
recognition.
Concentration:
1 mg/ml
Formulation:
100ug IgG in 100ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 496 Mouse: 11945 Rat: 24217
Immunogen Translated:
Hybridoma, fusion of spleen cells Balb / c nu removal of thymus cells and induce Sp2/0-Ag14 myeloma, autoimmune gastritis / + mouse neonatal
Reactivity:
This antibody reacts with human,
mouse, rat and bovine H
+
, K
+
-ATPase
α
subunit.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 2B6) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell Sp2/0-Ag14 with Balb/c nu/+ mouse splenocyte with
neonatal thymectomy-induced autoimmune gastritis.
Target:
Proton pump
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