Anti-Bcl-2 (Rat) mAb
| Code | Size | Price |
|---|
| MBL-D038-3 | 100 ug | £296.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 83-8B
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Flow Cytometry
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system) FCM - 10 ug/mL
Background:
The Bcl-2 related genes can inhibit
(Bcl-X
L
and Mcl-1) or induce (Bax, Bcl-Xs, Bag and Bad)
apoptosis in several systems. Bad was identified as a Bcl-2
interacting protein using a yeast two-hybrid screening and
?
expression cloning. It has homology to Bcl-2 within the
Bcl-2 homolog domains 1 and 2 (BH1 and BH2). In
mammalian cells, Bad selectively heterodimerizes with
Bcl-X
L
as well as Bcl-2, but not with other Bcl-2 family
members (Bax, Bcl-Xs, Mcl-1 and A1). When Bad
heterodimerized with Bcl-X
L
, it displaced Bax from Bcl-X
L
and promoted cell death.
Concentration:
1 mg/mL
Formulation:
100ug IgG in 100ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 596 Mouse: 12043
Immunogen Translated:
Rat recombinant protein Bcl-2beta
Reactivity:
This antibody reacts with human, mouse
and rat Bcl-2 on Western blotting and Flow cytometry.
Shelf Life:
1 year
Source:
This antibody was purified from mouse ascites
fluid using protein A agarose. This hybridoma was
established by fusion of
mouse myeloma cell PAI with
Balb/c mouse splenocyte immunized with recombinant rat
Bcl-2β
Target:
Bcl-2
References
?Viollet, L., et al., J. Immunol. 177, 6685-6694 (2006)
Tanaka, T., et al., Eur. Respir. J. 20 , 359-368 (2002)
Tamatani, M., et al., J. Biol. Chem. 274, 8531-8538 (1999) ?Nunez, G., et al., J. Immunol.144, 3602-3610 (1990)
Tsujimoto, Y., et al., Oncogene 4, 1331-1336 (1989)
Vaux, D. L., et al., Nature 335, 440-442 (1988)
Tsujimoto, Y., et al., Science 228, 1440-1443 (1985)