Anti-IL-18 (Human) mAb
Code | Size | Price |
---|
MBL-D043-3 | 100 ul | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 25-2G
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Images
Documents
Further Information
Applications:
WB - 1 ug/mL IHC - It is reported that this monoclonal antibody can be used in Immunohistochemistry for paraffin section in the reference number 2), and for frozen section in the reference number 1).
Background:
Interleukin 18 (IL
-
18) is a
n
18
kDa
cytokine which identified as a c
ostimulatory factor for
production of interferon
-
γ
(IFN
-
γ
) in response to toxic
shock and shares functional similarities with IL
-
12. IL
-
18
is
synthesized as a precursor 24 kDa molecule without a
signal
peptide and must be cleaved to produce an active
molec
ule.
IL
-
1 converting enzyme (ICE, Caspase
-
1)
cleaves pro
-
IL
-
18
at aspartic acid in the P1 position,
producing the mature,
bioactive peptide that is readily
released from the cells. It is
reported that IL
-
18 is produced
from Kupffer cells,
activated macroph
ages, keratinocytes,
intestinal epithelial
cells,
osteoblasts, adrenal cortex cells
and murine
diencephalon.
IFN
-
γ
is produced by activated T
or NK cells and plays critical roles in the defense against
microbiral pathogens.
IFN
-
γ
activates macrophages,
enhances NK activity and B
cell maturation, proliferation
and Ig secretion, induces MHC
class I and II antigens, and
inhibits osteoclast activation.
IL
-
18 acts on T helper
type
-
1
(Th1)
T cells and in combination
with IL
-
12 strongly
induces them to produce IFN
-
γ
.
Pleiotropic effects of IL
-
18
has also been reported, such as,
enhancement production of
IFN
-
γ
and GM
-
CSF in
p
eripheral blood mononuclear cells,
production of Th1 cytokines, IL
-
2, GM
-
CSF and IFN
-
γ
in
T cells,
enhancement of Fas ligand expression by Th1 cells.
Concentration:
1 mg/mL
Formulation:
100ug IgG in 100ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 3606 Mouse: 16173
Immunogen Translated:
Recombinant human IL-18
Reactivity:
This antibody
reacts with human IL
-
18
on Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from mouse ascites
fluid using protein
A agarose. This hybridoma was
established by fusion of mouse myeloma cell SP2/0 with
Balb/c mouse splenocyte immunized with recombinant
human IL
-
18.
Target:
IL-18
References
?1) Sakai, A., et al., J. Immunol. 181, 2898-2906(2008)
2) Takiyama, Y., et al., Thyroid 12, 935-943 (2002)
3) Shida, K., et al., J. Immunol. 166, 6671-6679 (2001)
4) Akita, K., et al., J. Biol. Chem. 272, 26595-26603 (1997)
5) Dao, T., et al., Cell Immunol. 173, 230-235 (1996)
6) Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)
7) Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)
8) Okamura, H., et al., Nature 378, 88-91 (1995)