Anti-IL-18 (Human) mAb-Biotin bulk_10mg
Code | Size | Price |
---|
MBL-D045-6L1T | 10 mL | £45,843.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 159-12B
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
ELISA - dilute 1:1,000
Background:
Interleukin 18 (IL
-
18) is a
n
18
kDa
cytokine which identified as a c
ostimulatory factor for
production of interferon
-
γ
(IFN
-
γ
) in response to toxic
shock and shares functional similarities with IL
-
12. IL
-
18
is
synthesized as a precursor 24 kDa molecule without a
signal
peptide and must be cleaved to produce an active
molec
ule.
IL
-
1 converting enzyme (ICE, Caspase
-
1)
cleaves pro
-
IL
-
18
at aspartic acid in the P1 position,
producing the mature,
bioactive peptide that is readily
released from the cells. It is
reported that IL
-
18 is produced
from Kupffer cells,
activated macroph
ages, keratinocytes,
intestinal epithelial
cells,
osteoblasts, adrenal cortex cells
and murine
diencephalon.
IFN
-
γ
is produced by activated T
or NK cells and plays critical roles in the defense against
microbiral pathogens.
IFN
-
γ
activates macrophages,
enhances NK activity and B
cell maturation, proliferation
and Ig secretion, induces MHC
class I and II antigens, and
inhibits osteoclast activation.
IL
-
18 acts on T helper
type
-
1
(Th1)
T cells and in combination
with IL
-
12 strongly
induces them to produce IFN
-
γ
.
Pleiotropic effects of IL
-
18
has also been reported, such as,
enhancement production of
IFN
-
γ
and GM
-
CSF in
p
eripheral blood mononuclear cells,
production of Th1 cytokines, IL
-
2, GM
-
CSF and IFN
-
γ
in
T cells,
enhancement of Fas ligand expression by Th1 cells.
Concentration:
500 ug/ml
Conjugate:
Biotin
Formulation:
25ug IgG in 50ul volume of PBS containing 1% BSA and 0.09% NaN3
Gene IDs:
Human: 3606 Mouse: 16173
Immunogen Translated:
Recombinant human IL-18
Reactivity:
This antibody
reac
ts with human IL
-
18
on ELISA
.
Shelf Life:
1 year
Source:
This antibody was purified from mouse ascites
fluid using protein
A agarose. This hybridoma was
established by fusion of mouse myeloma cell SP2/0 with
Balb/c mouse splenocyte immunized with recombinant
human IL
-
18.
Target:
IL-18
References
1)
McCurnin, D.,
et
al.
,
Pediatrics
121
, 945
-
956 (2008)
2)
Haveman, L. M.,
et al.
,
Int. Immunol.
18
, 1521
-
1529
(2006)
3)
Shigehara, K.,
et al
.,
J. Immunol.
166
, 642
-
649 (2001)
4)
Dao, T
.,
et al.
Cell Immunol.
173
, 230
-
235 (1996)
5)
Micallef,
M
.,
et al
.
Eur. J. Immunol.
2
6
, 1647
-
1651 (199
6)
6)
Ushio,
S
.,
et al.
J. Immunol.
156
, 4274
-
4279 (1996)
7)
Okamura, H
.,
et al
.
Nature
378
, 88
-
91 (1995)