Anti-IL-18 (Mouse) mAb
Code | Size | Price |
---|
MBL-D046-3 | 100 ul | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 39-3F
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Neutralisation
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
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Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system) NT - It is reported that this monoclonal antibody can be used in Neutralization in the reference number 5).
Background:
Interleukin 18 (IL
-
18) is a
n
18
kDa
cytokine which identified as a c
ostimulatory factor for
production of interferon
-
γ
(IFN
-
γ
) in response to toxic
shock and shares functional similarities with IL
-
12. IL
-
18
is
synthesized as a precursor 24 kDa molecule without a
signal
peptide and must be cleaved to produce an active
molec
ule.
IL
-
1 converting enzyme (ICE, Caspase
-
1)
cleaves pro
-
IL
-
18
at aspartic acid in the P1 position,
producing the mature,
bioactive peptide that is readily
released from the cells. It is
reported that IL
-
18 is produced
from Kupffer cells,
activated macroph
ages, keratinocytes,
intestinal epithelial
cells,
osteoblasts, adrenal cortex cells
and murine
diencephalon.
IFN
-
γ
is produced by activated T
or NK cells and plays critical roles in the defense against
microbiral pathogens.
IFN
-
γ
activates macrophages,
enhances NK activity and B
cell maturation, proliferation
and Ig secretion, induces MHC
class I and II antigens, and
inhibits osteoclast activation.
IL
-
18 acts on T helper
type
-
1
(Th1)
T cells and in combination
with IL
-
12 strongly
induces them to produce IFN
-
γ
.
Pleiotropic effects of IL
-
18
has also been reported, such as,
enhancement production of
IFN
-
γ
and GM
-
CSF in
p
eripheral blood mononuclear cells,
production of Th1 cytokines, IL
-
2, GM
-
CSF and IFN
-
γ
in
T cells,
enhancement of Fas ligand expression by Th1 cells.
Concentration:
1 mg/mL
Formulation:
100ug IgG in 100ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 3606 Mouse: 16173
Immunogen Translated:
Recombinant mouse IL-18
Reactivity:
This antibody
reacts with m
ouse IL
-
18
on Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from mouse ascites
fluid using protein
A agarose. This hybridoma was
established by fusion of mouse myeloma cell SP2/0 with
Balb/c mouse splenocyte immunized with recombinant
human IL
-
18.
Target:
IL-18
References
1)
Venkatachalam, K.,
et al
.,
J. Biol. Chem.
284
,
7853
-
7865 (2009) [WB]
2)
Hoshino, T.,
et al
.,
Am. J. Respir. Crit. Care Med.
176
49
-
62 (2007)
[WB]
3)
Seino, H.,
et al
.,
Am. J. Physiol. Gastrointest. Liver
Physiol.
292
, G262
-
G267 (2007)
[WB]
4)
Hoshino, T.,
et al
.,
J. Immunol.
166
, 701
4
-
7018 (2001)
[WB]
5)
Lertmemongkolchai, G.,
et al
.,
J. Immunol.
166
,
1097
-
1105 (2001)
[Neutralization]
6)
Dao, T
.,
et al.
Cell Immunol.
173
, 230
-
235 (1996)
7)
Ushio,
S
.,
et al.
J. Immunol.
156
, 4274
-
4279 (1996)
8)
Micallef,
M
.,
et al
.
Eur. J. Immunol.
2
6
, 1647
-
1651 (
1996)
9)
Okamura, H
.,
et al
.
Nature
378
, 88
-
91 (1995)
This clone is used in the reference number 1)
-
5).