Anti-IL-18 (Mouse) mAb bulk_50mg
Code | Size | Price |
---|
MBL-D048-3L5V | 50 mg | £159,604.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 93-10C
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Immunoprecipitation (IP)
- Neutralisation
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
IP - 5 ug/0.5 ug of recombinant mouse IL-18 NT - Induction of IFN-γ by mouse IL-18 receptor transfected KG-1 cell (KG-1 cell: Human myelomonocyte: ATCC CCL246) in response to the 30 ng/mL recombinant mouse IL-18 was neutralized by this antibody. The neutralization activity of 010 is as follows; Antibody concentration Inhibition dose* 0.5ug/mL > 50% 5ug/mL > 90%*Neutralization activity can be varied depends on cell conditions, IL-18 concentration.
Background:
Interleukin 18 (IL
-
18) is a
n
18
kDa
cytokine which identified as a c
ostimulatory factor for
production of interferon
-
γ
(IFN
-
γ
) in response to toxic
shock and shares functional similarities with IL
-
12. IL
-
18
is
synthesized as a precursor 24 kDa molecule without a
signal
peptide and must be cleaved to produce an active
molec
ule.
IL
-
1 converting enzyme (ICE, Caspase
-
1)
cleaves pro
-
IL
-
18
at aspartic acid in the P1 position,
producing the mature,
bioactive peptide that is readily
released from the cells. It is
reported that IL
-
18 is produced
from Kupffer cells,
activated macroph
ages, keratinocytes,
intestinal epithelial
cells,
osteoblasts, adrenal cortex cells
and murine
diencephalon.
IFN
-
γ
is produced by activated T
or NK cells and plays critical roles in the defense against
microbiral pathogens.
IFN
-
γ
activates macrophages,
enhances NK activity and B
cell maturation, proliferation
and Ig secretion, induces MHC
class I and II antigens, and
inhibits osteoclast activation.
IL
-
18 acts on T helper
type
-
1
(Th1)
T cells and in combination
with IL
-
12 strongly
induces them to produce IFN
-
γ
.
Pleiotropic effects of IL
-
18
has also been reported, such as,
enhancement production of
IFN
-
γ
and GM
-
CSF in
p
eripheral blood mononuclear cells,
production of Th1 cytokines, IL
-
2, GM
-
CSF and IFN
-
γ
in
T cells,
enhancement of Fas ligand expression by Th1 cells.
Concentration:
1 mg/mL
Formulation:
100ug IgG in 100ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 3606 Mouse: 16173
Immunogen Translated:
Recombinant mouse IL-18
Reactivity:
This antibody reacts
with mouse IL-18
on Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from mouse ascites
fluid using protein
A agarose. This hybridoma was
established by fusion of mouse myeloma cell SP2/0 with
Balb/c mouse splenocyte immunized with recombinant
human IL
-
18.
Target:
IL-18
References
1) Dao, T.,
et al., Cell Immunol.
173
, 230-235 (1996)
2) Micallef, M.,
et al
.,
Eur. J. Immunol
.
26
, 1647-1651 (1996)
3) Ushio, S.,
et al
.,
J. Immunol
.
156
, 4274-4279 (1996)
4) Okamura, H.,
et al
.,
Nature
378
, 88-91 (1995)