Anti-IL-18 (Mouse) mAb-Biotin bulk_50mg
Code | Size | Price |
---|
MBL-D048-6L1V | 50 mL | £327,295.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 93-10C
Regulatory Status: RUO
Target Species: Mouse
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
ELISA - dilute 1:2,000
Background:
Interleukin 18 (IL
-
18) is a
n
18
kDa
cytokine which identified as a c
ostimulatory factor for
production of interferon
-
γ
(IFN
-
γ
) in response to toxic
shock and shares functional similarities with IL
-
12. IL
-
18
is
synthesized as a precursor 24 kDa molecule without a
signal
peptide and must be cleaved to produce an active
molec
ule.
IL
-
1 converting enzyme (ICE, Caspase
-
1)
cleaves pro
-
IL
-
18
at aspartic acid in the P1 position,
producing the mature,
bioactive peptide that is readily
released from the cells. It is
reported that IL
-
18 is produced
from Kupffer cells,
activated macroph
ages, keratinocytes,
intestinal epithelial
cells,
osteoblasts, adrenal cortex cells
and murine
diencephalon.
IFN
-
γ
is produced by activated T
or NK cells and plays critical roles in the defense against
microbiral pathogens.
IFN
-
γ
activates macrophages,
enhances NK activity and B
cell maturation, proliferation
and Ig secretion, induces MHC
class I and II antigens, and
inhibits osteoclast activation.
IL
-
18 acts on T helper
type
-
1
(Th1)
T cells and in combination
with IL
-
12 strongly
induces them to produce IFN
-
γ
.
Pleiotropic effects of IL
-
18
has also been reported, such as,
enhancement production of
IFN
-
γ
and GM
-
CSF in
p
eripheral blood mononuclear cells,
production of Th1 cytokines, IL
-
2, GM
-
CSF and IFN
-
γ
in
T cells,
enhancement of Fas ligand expression by Th1 cells.
Conjugate:
Biotin
Formulation:
In 50ul volume of PBS containing1% BSA, 10% glycerol and 0.09%sodiumazide, pH 7.2.*Azide may react with copper or lead in plumbing system to form explosive metalazides.Therefore, always flush plenty ofwater when disposing materials containing a
Gene IDs:
Human: 3606 Mouse: 16173
Immunogen Translated:
Recombinant mouse IL-18
Reactivity:
This antibody reacts with mouse IL
-
18
on ELISA.
Shelf Life:
1 year
Source:
This antibody was purified from
hybridoma
(clone
93
-
10C
) supernatant
using protein G agarose
.
Thi
s
hybridoma
was established
by fusion of mouse myeloma
cell
SP2/0
with SD rat splenocyte immunized with
recombinant mouse IL
-
18.
Target:
IL-18
References
1) Dao, T.,
et al., Cell Immunol.
173
, 230-235 (1996)
2) Micallef, M.,
et al
.,
Eur. J. Immunol
.
26
, 1647-1651 (1996)
3) Ushio, S.,
et al
.,
J. Immunol
.
156
, 4274-4279 (1996)
4) Okamura, H.,
et al
.,
Nature
378
, 88-91 (1995)