Anti-Multi Ubiquitin mAb
| Code | Size | Price |
|---|
| MBL-D058-3 | 100 ug | £363.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: FK2
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1-5 ug/mL for chemiluminescence
detection system
Background:
Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. Recently several reports showed that intracellular abnormal and short-lived proteins are degradated through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
Immunogen Translated:
Lysozyme - poly-ubiquitin was purified crude
Reactivity:
This antibody reacts with multi
ubiquitin chains, but it doesn?t react with mono ubiquitin
and free ubiquitin on Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from ascites fluid using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell with Balb/c mouse splenocyte immunized with a crude
poly-ubiquitin-lysozyme.
Target:
Ubiquitin
References
1) Ishioka, T., et al., Genes Cells 12, 735-744 (2007)
2) Hosokawa, H., et al., J. Immunol. 177, 7656-7664 (2006)
3) Masuda, Y., et al., J. Lipid Res. 47, 87-98 (2006)
4) Nakamura, M., et al., Genes Cells 11, 593-606 (2006)
5) Yamashita, M., et al., J. Biol. Chem. 280, 29409-29419 (2005)
6) Hwang, G-W., et al., Mol. Pharmacol. 68, 1074-1078 (2005)
7) Broering, T. J., et al., J. Virol. 79, 6194-6206 (2005)
8) Inukai, N., et al., J. Biol. Chem. 279, 8190-8195 (2004)
9) Katoh, K., et al., J. Biol. Chem. 278, 39104-39113 (2003)
10) Seino, H., et al., Mol. Cell Biol. 23, 3497-3505 (2003)
11) Yokosawa, N., et al., J. Virol. 76, 12683-12690 (2002)
12) Takada, K., et al., Eur. J. Biochem. 233, 42-47 (1995)
13) Fujimuro, M., et al., FEBS Lett. 349, 173-180 (1994)
Clone FK2 is used in these references.