Anti-Multi Ubiquitin mAb-Agarose
| Code | Size | Price |
|---|
| MBL-D058-8 | Gel: 200 uL | £433.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: FK2
Regulatory Status: RUO
Target Species: Human
Application: Immunoprecipitation (IP)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
IP - 20 uL/300 uL of cell extract from 3x106 cells
Background:
Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. Recently several reports showed that intracellular abnormal and short-lived proteins are degradated through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules.
Conjugate:
Agarose
Formulation:
500-600 ug of anti-Multi Ubiquitin monoclonal antibody covalently coupled to 200 uL of agarose gel and provided as a 50% gel slurry (in PBS/0.1% ProClin 150)
Immunogen Translated:
Lysozyme - poly-ubiquitin was purified crude
Reactivity:
It is reported clone FK2 recognizes
K29-, K48-, K63-linked poly ubiquitinylated and mono
ubiquitinylated proteins but not free ubiquitin in on
Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from ascites fluid using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell with Balb/c mouse splenocyte immunized with a crude poly-ubiquitin-lysozyme.
Target:
Ubiquitin
References
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