Anti-Multi Ubiquitin mAb
| Code | Size | Price |
|---|
| MBL-D071-3 | 100 ug | £366.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgM
Antibody Clonality: Monoclonal
Antibody Clone: FK1
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1-5 ug/mL (chemiluminescence detection system)
Background:
Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. There are several reports showed that intracellular abnormal and short-lived proteins are degraded through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules.
Concentration:
1 mg/ml
Formulation:
100 ug IgM in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Immunogen Translated:
Lysozyme - poly-ubiquitin was purified crude
Reactivity:
It is reported Clone FK1 recognizes K29-, K48-, K63-linked poly ubiquitinylated proteins but not mono ubiquitinylated proteins or free ubiquitin on Western blotting in the reference number 1).
Shelf Life:
1 year
Source:
This antibody was purified from mouse ascites fluid using protein L agarose. This hybridoma (clone FK1) was established by fusion of mouse myeloma cell with Balb/c mouse splenocyte immunized with the crude poly-ubiquitin-lysozyme.
Target:
Ubiquitin
References
1)
Morris, J. R., and Solomon, E., Hum. Mol. Genet. 13, 807-817 (2004)
2)
Seino, H., et al., Mol. Cell Biol. 23, 3497-3505 (2003)
3)
Lyakhovich, A., et al., Mol. Cell Biol. 23, 2463-2475 (2003)
4)
Takeuchi, J., et al., Mol. Cell Biol. 19, 6575-6584 (1999)
5)
Takada, K., et al., Eur. J. Biochem. 233, 42-47 (1995)
6)
Fujimuro, M., et al., FEBS. Letters. 349, 173-180 (1994)
Clone FK1 is used in reference number 1) - 4) and 6).