Anti-HMGB1(HMG1) (Pig) mAb

WB - 5 ug/mL (chemiluminescence detection system)

High mobility group box 1 (HMGB1), named for its rapid migration properties on electrophoretic gels, is a member of the nonhistone chromatin-associated proteins. HMGB1 is translated as a 214 amino acid protein and is extensively modified posttranslationally by glycosylation, acylation, methylation, and phosphorylation. The primary structure is evolutionarily conserved, with 100% amino acid sequence homology between rat and mouse and 99% homology between rodent and human. Intracellular HMGB1 has previously been studied for its roles in binding DNA; stabilizing nucleosome formation; as a general transcription factor for nucleolar and mitochondrial RNA polymerases; and as a gene- and tissue-specific transcriptional regulator that can enhance transcription and/or replication. Extracellular HMGB1 has recently been implicated as a late mediator of delayed endotoxin lethality, because murine and human macrophages/monocytes release large amounts of a 29 kDa form of HMGB1 when stimulated by exposure to bacterial endotoxin.

1 mg/ml

100 ug IgG in a 100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains nopreservatives.

Human: 3146 Mouse: 15289

Pig HMG1

This antibody reacts with HMGB1 (29 kDa) on Western blotting.

1 year

This antibody was purified from hybridoma (clone KS1) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell PAI with Balb/c mouse lymphocyte immunized with porcine HMGB1.

HMGB1