Anti-TLR4-MD-2 complex (Mouse) mAb
| Code | Size | Price |
|---|
| MBL-D079-3 | 100 ug | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: MTS510
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Flow Cytometry
- Immunoprecipitation (IP)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
IP - It is reported that clone MTS510 can be used in this application FCM - 10-20 ug/mL NT - It is reported that clone MTS510 can be used in this application
Background:
TLR4 (Toll-like receptor 4), a
member of Toll-like receptor family, may act as a receptor
for LPS (lipopolysaccharide). It associates with the MD-2
molecule by its extracellular domain. The LPS signal
through TLR4 may be greatly enhanced by this association.
This antibody (clone MTS510) reacts selectively with
mouse TLR4-MD-2 complex but not with TLR4 alone in
flow cytometry.
Concentration:
1 mg/ml
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol. No preservative iscontained.
Gene IDs:
Human: 7099 Mouse: 21898
Immunogen Translated:
Ba/F3 cells expressing mouse TLR4-MD-2 complex
Reactivity:
This antibody reacts with mouse
TLR4-MD-2 complex on Flow cytometry. This antibody
doesn?t react with mouse TLR4 alone.
Shelf Life:
1 year
Source:
This antibody was purified from culture
supernatant using protein G agarose. This hybridoma was
established by fusion of mouse myeloma cell SP2/0 with
Wister rat lymph node cells immunized with mouse Ba/F3
cells expressing mouse TLR4-MD-2 complex.
Target:
TLR4-MD2
References
1) Wakabayashi, Y., et al., J. Immunol. 177, 1772-1779 (2006)
2) Kobayashi, M., et al., J. Immunol. 176, 6211-6218 (2006)
3) Akashi-Takamura, S., et al., J. Immunol. 176, 4244-4251
(2006)
4) Saitoh, S., et al., Int. Immunol. 16, 961-969 (2004)
5) Akashi, S., et al., J. Exp. Med. 198, 1035-1042 (2003)
6) Yazawa, N., et al., Blood 102, 1374-1380 (2003)
7) Kawasaki, K., et al., J. Immunol. 170, 413-420 (2003)
8) Nagai, Y., et al., Nat. Immunol. 3, 667-672 (2002)
9) Akashi, S., et al., Int. Immunol. 13, 1595-1599 (2001)
10) Ogata, H., et al., J. Exp. Med. 192, 23-30 (2000)
11) Nomura, F., et al., J. Immunol. 164, 3476-3479 (2000)
12) Akashi, S., et al., J. Immunol. 164, 3471-3475 (2000)
13) Akashi, S., et al., Biochem. Biophys. Res. Commun. 268,
172-177 (2000)
14) Shimazu, R., et al., J. Exp. Med. 189, 1777-1782 (1999)
Clone MST510 is used in reference number 1) - 12).