Anti-ASC (TMS1) (Human) mAb
| Code | Size | Price |
|---|
| MBL-D086-3 | 100 ug | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 23-4
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1 ug/mL (chemiluminescence detection system) IP - 5 ug/300 uL of cell extract from 5x106 HL-60 cells ICC - It is reported that this monoclonal antibody can be used in immunocytochemistry in the reference number 11). IHC - It is reported that this monoclonal antibody can be used in immunohistochemistry in the reference number 8).
Background:
ASC (apoptosis-associated speck-like protein containing a CARD (caspase recruitment domain)) is a 22 kDa soluble protein, located in the cytosol of HL-60 cells. In apoptotic HL-60 cells, it is able to visualized as a speck, forming an insoluble aggregate. The C-terminal domain of this protein contains a CARD, suggesting that ASC may have proapoptotic activity in HL-60 cells. Recent data have indicated that ASC plays a role of adaptor protein linking various PAAD (Pyrin, AIM, ASC, and death domain-like) family proteins to pathways involved in NF-κB and procaspase-1 activation.
Formulation:
100 ug IgG in a 100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains nopreservatices.
Gene IDs:
Human: 29108 Mouse: 66824
Immunogen Translated:
TritonX-100 insoluble fraction of HL-60 cells were treated with retinoic acid
Reactivity:
This antibody reacts with human ASC (22 kDa) on Western blotting and Immunoprecipitation.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 23-4) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3-X63-AG8.653 with Balb/c mouse splenocyte immunized with Triton X-100 insoluble fraction of retinoic acid-treated HL-60 cells.
Target:
ASC
References
1)
Bryan, N. B., et al., J. Inflamm. (Lond.) 7, 23 (2010)
2)
Mayor, A., et al., Nat. Immunol. 8, 497-503 (2007)
3)
Hasegawa, M., et al., J. Biol. Chem. 280, 15122-15130 (2005)
4)
Terasawa, K., et al., Clin. Cancer Res. 10, 2000-2006 (2004)
5)
Wang, Y., et al., Int. Immunol. 16, 777-786 (2004)
6)
Stehlik, C., et al., J. Immunol. 171, 6154-6163 (2003)
7)
Stehlik, C., et al., J. Exp. Med. 196, 1605-1615 (2002)
8)
Masumoto, J., et al., J. Histochem. Cytochem. 49, 1269-1275 (2001)
9)
Masumoto, J., et al., Exp. Cell Res. 262, 128-133 (2001)
10)
Masumoto, J., et al., Biochem. Biophys. Res. Commun. 280, 652-655 (2001)
11)
Masumoto, J., et al., J. Biol. Chem. 274, 33835-33838 (1999)
Clone 23-4 is used in these references.