Anti-GPI-80 (Human) mAb
| Code | Size | Price |
|---|
| MBL-D087-3 | 100 ug | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3H9
Regulatory Status: RUO
Target Species: Human
Applications:
- Flow Cytometry
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1-5 ug/mL (chemiluminescence detection system) FCM - 1-10 ug/mL (final concentration)
Background:
The GPI-80 molecule (80 kDa)
recognized by this antibody (clone 3H9) was shown to be
present on human neutrophils. When 3H9 was added with a
neutrophil stimulant (fMLP), the inhibition of neutrophil
adherence was observed after 60 minutes incubation. 3H9
enhanced not only fMLP-induced chemotaxis but random
migration of neutrophil as well. Furthermore, 3H9 clearly
discriminated neutrophils from both basophils and
eosinophils derived from humans.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
Gene IDs:
Human: 8875
Immunogen Translated:
Human neutrophils activated with PMA
Reactivity:
This antibody reacts with human GPI-80
on Western blotting and Flow cytometry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 3H9) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell X63-Ag8.653 with Balb/c mouse splenocyte
immunized with PMA activated human neutrophil.
Target:
GPI-80/VNN2
References
1) Sendo, D., et al., Yamagata Med. J. 23, 69-82 (2005)
2) Yoshitake, H., et al., J. Leukoc. Biol. 71, 205-211 (2002)
3) Dahlgren, C., et al. J. Leukoc. Biol. 69, 57-62 (2001)
4) Huang, J., et al. Microbiol. Immunol. 45, 467-71 (2001)
5) Nakamura-Sato, Y., et al., J. Leukoc. Biol., 68, 650-654 (2000)
6) Suzuki, K., et al. J. Immunol. 162, 4277-84 (1999)
7) Ohtake, K., et al., Microbiol. Immunol. 41, 67-72 (1997)
Clone 3H9 is used in these references.