Anti-CD274 (PD-L1) (Human) mAb

FCM - 5-10 ug/mL (final concentration)

Programmed death ligand 1 (PD-L1, also known as CD274/B7-H1), a member of B7 family was identified by searching for molecules that share homology with the immunoglobulin V and C domains of B7-1 and B7-2 among the human cDNA expressed sequence tags in the National Center for Biotechnology Information database. PD-L1 is a ligand for programmed death 1 (PD-1) which belongs to the CD28/CTLA4 subfamily. Although in vitro study indicated that the cross-linking of PD-1 by PD-L1 leads to down-regulation of T-cell responses, some studies have shown that T cells stimulated with low levels of anti-CD3 and immobilized PD-L1-Ig were activated, proliferation and production of IFN-γ GM-CSF and IL-10 from the T cells were enhanced.

1 mg/mL

1.0 mg/mL in PBS/50% glycerol, pH 7.2

Human: 29126 Mouse: 60533

L cells were transfected with full-length human PD-L1

This antibody reacts with CD274 antigen on Flow cytometry.

1 year

This antibody was purified from hybridoma (clone MIH3) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell SP2/0 with Balb/c mouse splenocyte immunized with the full-length human PD-L1 transfected L cells.

CD274