Anti-Phosphorylated Vimentin (Ser82) mAb
| Code | Size | Price |
|---|
| MBL-D095-3 | 100 ug | £350.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: MO82
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunocytochemistry (ICC)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 0.1 ug/mL (chemiluminescence detection system) ICC - 0.2 ug/mL
Background:
Components of intermediate
filaments provide information on the origin of vertebrate
cells. Antibody to vimentin can be used as to identify the
vimentin subclass of intermediate filaments. Vimentin is a
~58 kDa, widely expressed protein that thought to be
involved mainly in structural processes, such as wound
healing. Scientists have also recently determined that
activated human macrophages secrete vimentin into the
extracellular space, and overproduction of vimentin has
been associated with cellular senescence.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
Gene IDs:
Human: 7431 Mouse: 22352
Immunogen Translated:
PV82 peptide KLH-phosphorus oxide (CRLLQDpSVDFSL)
Reactivity:
This antibody recognizes the
site-specific phosphorylation of vimentin at Ser82 on
Western blotting and Immunocytochemistry.
Shelf Life:
1 year
Source:
This antibody was purified from mouse ascites
fluid using protein A agarose. This hybridoma (clone
MO82) was established by fusion of mouse myeloma cell
SP2/0-Ag14 with Balb/c mouse splenocyte immunized with
the KLH conjugated phospho-peptide PV82
(CRLLQDpSVDFSL).
Target:
Vimentin
References
1) Oguri, T., et al., Genes Cells 11, 531-540 (2006)
2) Tsui, J., and Malenka R. C., J. Biol. Chem. 281, 13794-13804 (2006)
3) Yamaguchi, T., et al., J. Cell Biol. 171, 431-436 (2005)
4) Stefanovic, S., et al., J. Virol. 79, 11766-11775 (2005)
5) Tsui, J., et al., J. Biol. Chem. 280, 9210-9216 (2005)
6) Harvey, B. P., et al., J. Biol. Chem. 279, 24889-24898 (2004)
7) Nagata, K., et al., Genes Cells 6, 653-664 (2001)
8) Inagaki, N., et al., J. Biol. Chem. 275, 27165-27171 (2000)
9) Inada, H., et al., J. Biol. Chem. 274, 34932-34939 (1999)
10) Usui, T., et al., J. Biochem. (Tokyo) 125, 960-965 (1999)
11) Kitani, A., et al., J. Immunol. 161, 4931-4938 (1998)
12) Goto, H., et al., J. Biol. Chem. 273, 11728-11736 (1998)
13) Inagaki, N., et al., J. Biol. Chem. 272, 25195-25199 (1997)
14) Tsujino, S., et al., BBRC 219, 633-637 (1996)
15) Ogawara, M., et al., J. Cell Biol. 131, 1055-1066 (1995)
Clone MO82 is used in these references.