Anti-CENP-A (Human) mAb

Product Code: MBL-D115-3

Supplier: MBL

Code	Size	Price

MBL-D115-3

100 ug

£336.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgG1

Antibody Clonality: Monoclonal

Antibody Clone: 3-19

Regulatory Status: RUO

Target Species: Human

Applications:

Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
Western Blot (WB)

Shipping:

4°C

Storage:

-20°C

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Documents

Datasheet

Further Information

Applications:

WB - 1 ug/mL ICC - 10 ug/mL IHC - 5 ug/mL Heat treatment is necessary for paraffin embedded sections. Microwave oven; 2 times for 10 minutes each in 10 mM citrate buffer (pH 6.5) Other - It is reported that this monoclonal antibody can be used in Chromatin immunoprecipitation (ChIP) in the reference number 3) and 4).

Background:

Centromere protein A (CENP-A) is a 17-19 kDa centromere-specific histone variant that is 62% identical to the carboxy-terminal domain of histone H3. In the presence of DNA, CENP-A forms an octameric complex with histones H4, H2A, and H2B. CENP-A defines active centromere regions by forming centromere-specific nucleosomes on which kinetochores are assembled. CENP-A is specifically located to the inner plate of the kinetochore and it is essential for kinetochore targeting of CENP-C and other kinetochore components. CENP-A is required for nucleosomal packaging of centromeric DNA at interphase.

Concentration:

1 mg/mL

Formulation:

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Gene IDs:

Human: 1058 Mouse: 12615

Immunogen Translated:

Human CENP-A peptide (3-19 aa)

Reactivity:

This antibody reacts with human CENP-A (17 kDa) on Western blotting, Immunohistochemistry and Immunocytochemistry.

Shelf Life:

1 year

Source:

This antibody was purified from hybridoma (clone 3-19) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3-X63-Ag8 with Balb/c mouse splenocyte immunized with human CENP-A peptide (3-19 aa).

Target:

CENP-A

References

1) Hemmerich, P., et al., J. Cell Biol. 180, 1101-1114 (2008) 2) Chi, Y. H., et al., J. Biol. Chem. 282, 27447-27458 (2007) 3) Mimori-Kiyosue, Y., et al., Genes Cells 11, 845-857 (2006) 4) Izuta, H., et al., Genes Cells 11, 673-684 (2006) 5) Obuse, C., et al., Genes Cells 9, 105-120 (2004) 6) Ando, S., et al., Mol. Cell. Biol. 22, 2229-2241 (2002) Clone 3-19 is used in these references.