Anti-Sap155 (Mouse) mAb

WB - 1 ug/mL (chemiluminescence detection system)

SF3 is a U2 snRNP-associated protein complex essential for spliceosome assembly and splicing catalysis of the major spliceosome. SF3 contains the Spliceosome-Associated Proteins, SAP 49, 130, 145, and 155. SAP155/Sf3b1 is an essential subunit of the U2 snRNP for mRNA splicing and has also been identified in the minor (U12-dependent) spliceosome. SAP155 interacts with the mammalian PcG (Polycomb group) proteins, Mel18 and Ring1B by the yeast two-hybrid system. SAP155 contains numerous Cdk consensus phosphorylation sites in its N terminus and is phosphorylated prior to catalytic step II of the splicing pathway. SAP155 serves as a substrate for cyclin E-cdk2 in vitro, suggesting that pre-mRNA splicing may be linked to the cell cycle machinery in mammalian cells.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.

Human: 23451 Mouse: 81898

Recombinant mouse Sap155

This antibody reacts with human, mouse and hamster Sap155 on Western blotting.

1 year

This antibody was purified from hybridoma (clone 1A5) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with the recombinant mouse Sap155.

Sap155