Anti-Abi-1 (Abl interactor 1) (Human) mAb

WB - 1 ug/mL (chemiluminescence detection system) IP - 2 ug/200 uL of cell extract from 5x106 cells IHC - 5 ug / mL Heat treatment is necessary for paraffin embedded sections. Microwave oven; 2 times for 10 minutes each in citrate buffer (pH 6.5)

Abl interactor (Abi) proteins are c-Abl-binding proteins that bind to both the Src homology 3 (SH3) domain and the C-terminal proline-rich regions of Abl kinase through dual SH3-PXXP interactions. Two highly related genes, abi-1 and abi-2, were cloned. In addition to the interaction with Abl kinase, Abi proteins also interact with other signaling molecules. Recently, it has been reported that Abi-1 dramatically promoted c-Abl-mediated tyrosine (Tyr296) phosphorylation of Mena [mammalian homologue of Drosophila Enabled (Ena)] by interacting with both proteins.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.

Human: 10006 Mouse: 11308

Recombinant human Abi-1 was expressed in Escherichia coli

This antibody reacts with Abi-1 (65 kDa) on Western blotting.

3 years

This antibody was purified from hybridoma (clone 1B9) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp/O with Balb/c mouse splenocyte immunized with a human recombinant Abi-1 expressed in E. coli.

Abi1