Anti-GFP (Green Fluorescent Protein) mAb
| Code | Size | Price |
|---|
| MBL-D153-3 | 100 ul | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: RQ2
Regulatory Status: RUO
Applications:
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
IP - 0.5-2 ug/sample ICC - 2 ug/mL
Background:
Since the detection of intracellular Aequorea Victria Green Fluorescent Protein (GFP) requires only irradiation by UV or blue light, it provides an excellent means for monitoring gene expression and protein localization in living cells. Monoclonal anti-GFP antibody can detect GFP fusion protein on Immunoprecipitation.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Immunogen Translated:
GFP was affinity-purified anti-GFP by using a mouse from 293T cells expressing GFP
Reactivity:
This antibody reacts with GFP fusion protein on Immunoprecipitation and Immunocytochemistry. It reacts with EBFP, ECFP, EGFP, Venus and Sapphire
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone RQ2) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell PAI with Wister rat lymphonode immunized with GFP purified from GFP expressed 293T cells by affinity chromatographic technique using mouse anti-GFP.
Target:
GFP
References
1) Cai, L., et al., J. Biol. Chem. 286, 35915-35921 (2011)
2) Sato, Y., et al., J. Biol. Chem. 284, 11873-11881 (2009)
3) Sakurai, T., et al., J. Cell Biol. 183, 339-352 (2008)
4) Kato, A., et al., J. Virol. 82, 6172-6189 (2008)
5) Dragone, L. L., et al. PNAS. 103, 18202-18207 (2006)
6) Darzacq, X., et al. J. Cell Biol. 173, 207-218 (2006)
7) Hayakawa, T., et al. Plant Cell Physiol. 47, 891-904 (2006)
8) Obuse, C., et al. Nat. Cell Biol. 6, 1135-1141 (2004)