Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor 488

Product Code: MBL-D153-A48

Supplier: MBL

Code	Size	Price

MBL-D153-A48

50 ul

£268.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rat

Antibody Isotype: IgG2a κ

Antibody Clonality: Monoclonal

Antibody Clone: RQ2

Regulatory Status: RUO

Application: Immunocytochemistry (ICC)

Shipping:

4°C

Storage:

4°C

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Documents

Datasheet

Further Information

Applications:

ICC - 2-5 ug/mL *This antibody is not suitable for alcohol fixation such as ethanol or methanol.

Background:

Since the detection of intracellular Aequorea Victria Green Fluorescent Protein (GFP) requires only irradiation by UV or blue light, it provides an excellent means for monitoring gene expression and protein localization in living cells. Monoclonal anti-GFP antibody can detect GFP fusion protein on Immunocytochemistry.

Concentration:

1 mg/mL

Formulation:

50 ug IgG in 50 ul volume of PBS containing 1% BSA and 0.09% NaN3.*Azide may react with copper or lead in plumbing system to formexplosive metal azides. Therefore, always flush plenty of waterwhen disposing materials containing azide into drain.

Immunogen Translated:

GFP was affinity-purified anti-GFP by using a mouse from 293T cells expressing GFP

Reactivity:

This antibody reacts with GFP fusion protein on Immunocytochemistry. It reacts with EBFP, ECFP, EGFP, Venus and Sapphire.

Shelf Life:

1 year

Source:

This antibody was purified from hybridoma (clone RQ2) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell PAI with Wistar rat lymph node immunized with GFP purified from GFP expressed 293T cells by affinity chromatographic technique using mouse anti-GFP

Target:

GFP

References

1) Sato, Y., et al., J. Biol. Chem. 284, 11873-11881 (2009) 2) Sakurai, T., et al., J. Cell Biol. 183, 339-352 (2008) 3) Kato, A., et al., J. Virol. 82, 6172-6189 (2008) 4) Dragone, L. L., et al. PNAS. 103, 18202-18207 (2006) 5) Darzacq, X., et al. J. Cell Biol. 173, 207-218 (2006) 6) Hayakawa, T., et al. Plant Cell Physiol. 47, 891-904 (2006) 7) Obuse, C., et al. Nat. Cell Biol. 6, 1135-1141 (2004) Clone RQ2 is used in these references.