Anti-MFG-E8 (Mouse) mAb

IP - 5 ug/250 mL of mouse bone marrow cells culture supernatant FCM - 5-10 ug/mL (final concentration)

?Apoptotic cells are rapidly removed by phagocytosis to clear the living cells and tissues of intracellular materials being released from dying cells. Phagocytes recognize apoptotic cells through milk fat globule-EGF-factor 8 (MFG-E8). MFG-E8 is a secreted glycoprotein from thioglycollate-elicited macrophages and bind to phosphatidylserine exposed on the cell surface of apoptotic cells, then bind strongly to cells expressing α?β3 integrin via its RGD (arginine-glycine-aspartate) motif. Thus, MFG-E8 is an important protein to mediate the engulfment of apoptotic cells by activated macrophages.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 4240 Mouse: 17304

Mouse peritoneal macrophages were attracted by Thioglycollate

This antibody reacts with mouse MFG-E8 short and long form on Flow cytometry and Immunoprecipitation.

1 year

This antibody was purified from hybridoma (clone 2422) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell with Armenian hamster lymph node immunized with thioglycollate-elicited mouse peritoneal macrophages.

MFG-E8