Anti-Flavocytochrome b558 (Human) mAb

FCM - 5 ug/mL (final concentration)

The NADPH oxidase is a multicomponent enzyme that transfers electrons from NADPH to O2 to generate superoxide (O2 -), a key part of the phagocytic or neutrophilic respiratory burst response. Flavocytochrome b558 is the catalytic component of the phagocyte NADPH oxidase. It is a transmembrane heterodimer composed of a large glycoprotein, gp91phox (PHagocyte OXidase) and a smaller protein, p22phox. Upon cell stimulation, flavocytochrome B558 assembles with p67phox, p47phox, and the GTP-binding protein Rac and becomes activated to generate O2 -. Mutations in gp91phox, p22phox, or other components of the NADPH oxidase can result in chronic granulomatous disease, which is associated with significant morbidity and mortality due to a predisposition to recurrent bacterial and fungal infections.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 1535 Mouse: 13057

Rich fraction of human cytochrome b

This antibody reacts with an extracellular epitope of gp91phox. The antibody recognizes the epitope in the native conformation or after treatment with guanidine or urea, but SDS treatment will abolish antibody binding.

1 year

This antibody was purified from hybridoma (clone 7D5) supernatant using protein-A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0 with Balb/c mouse splenocyte immunized with the human cytochrome b rich fraction.

Flavocytochrome b