Anti-Flavocytochrome b558 (Human) mAb-FITC

FCM - 10 ug/mL (final concentration)

The NADPH oxidase is a multicomponent enzyme that transfers electrons from NADPH to O2 to generate superoxide (O2 -), a key part of the phagocytic or neutrophilic respiratory burst response. Flavocytochrome b558 is the catalytic component of the phagocyte NADPH oxidase. It is a transmembrane heterodimer composed of a large glycoprotein, gp91phox (PHagocyte OXidase) and a smaller protein, p22phox. Upon cell stimulation, flavocytochrome b558 assembles with p67phox, p47phox, and the GTP-binding protein Rac and becomes activated to generate O2 -. Mutations in gp91phox, p22phox, or other components of the NADPH oxidase can result in chronic granulomatous disease, which is associated with significant morbidity and mortality due to a predisposition to recurrent bacterial and fungal infections.

0.5 mg/mL

FITC

50 ug IgG in 100 ul volume of PBS containing 1% BSA and 0.09% NaN3.*Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush plenty ofwater when disposing materials containing azide into drain.

Human: 1535 Mouse: 13057

Rich fraction of human cytochrome b

This antibody reacts with an extracellular epitope of gp91phox. The antibody recognizes the epitope in the native conformation. This antibody reacts with Flavocytochrome b558 on flow cytometry.

1 year

This antibody was purified from hybridoma (clone 7D5) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0 with Balb/c mouse splenocyte immunized with the human cytochrome b rich fraction.

Flavocytochrome b