Anti-Flavocytochrome b558 (Human) mAb-PE
| Code | Size | Price |
|---|
| MBL-D162-5 | 50 Tests | £328.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 7D5
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
FCM - 20 uL (ready for use)
Background:
The NADPH oxidase is a
multicomponent enzyme that transfers electrons from
NADPH to O
2
to generate superoxide (O
2
-
), a key part of
the phagocytic or neutrophilic respiratory burst response.
Flavocytochrome b
558
is the catalytic component of the
phagocyte NADPH oxidase. It is a transmembrane
heterodimer composed of a large glycoprotein, gp91
phox
(PHagocyte OXidase) and a smaller protein, p22
phox
.
Upon cell stimulation, flavocytochrome b
558
assembles
with p67
phox
, p47
phox
, and the GTP-binding protein Rac
and becomes activated to generate O
2
-
. Mutations in
gp91
phox
, p22
phox
, or other components of the NADPH
oxidase can result in chronic granulomatous disease,
which is associated with
significant morbidity and
mortality due to a predisposition to recurrent bacterial and
fungal infections.
Conjugate:
PE
Gene IDs:
Human: 1535, 1536 Mouse: 13057
Immunogen Translated:
Rich fraction of human cytochrome b
Reactivity:
This antibody recognizes the
extracellular peptide portion of primate gp91
phox
of the
human Flavocytochrome b
558
on Flow cytometry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone 7D5) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell Sp2/0 with Balb/c mouse splenocyte immunized with
the human cytochrome b rich fraction.
Target:
Flavocytochrome b558
References
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