Anti-Granulysin (Human) mAb
| Code | Size | Price |
|---|
| MBL-D186-3 | 100 ug | £328.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: RF10
Regulatory Status: RUO
Target Species: Human
Applications:
- Flow Cytometry
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 1 ug/mL IP - 5 ug/200 uL of cell extract from 5x106 cells FCM - 5-10 ug/mL (final concentration) ICC - 2-5 ug/mL
Background:
Granulysin is an antimicrobial protein expressed on cytotoxic T cells, natural killer (NK) cells and NKT cells. It has been shown that Granulysin contributes to the defence mechanisms against variety of mycobacterial infection and tumors. Granulysin has two molecular forms, 15 kDa precursor and 9 kDa effector form. There serum levels were significantly elevated during the acute viral infections and correlated with the NK cell and CTL activities in patients with sever immunodeficiency, indicate that serum Granulysin could be useful novel marker to evaluate the overall status of host cell immunity.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
Gene IDs:
Human: 10578
Immunogen Translated:
Human full-length expression plasmid Guranyuraishin
Reactivity:
This antibody immunopricipitates Granulysin.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone RF10) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0-Ag8 with Balb/c mouse splenocyte immunized with the full-length human Granulysin expression plasmid.
Target:
Granulysin
References
1)
Merlo, A., et al., J. Immunol. 184, 5895-5902 (2010)
2)
Ogawa, K., et al., Eur. J. Immunol. 33, 1925-1933 (2003)
3)
Gamen, S., et al., J. Immunol. 161, 1758-1764 (1998)
Clone RF10 is used in reference number 1) and 2).