Anti-MFG-E8 (Mouse) mAb

Product Code:

MBL-D199-3

Product Group:

Primary Antibodies

Supplier:

MBL

Host Type:

Hamster

Antibody Isotype:

IgG

Antibody Clonality:

Monoclonal

Antibody Clone:

18A2-G10

Regulatory Status:

RUO

Target Species:

Mouse

Applications:

Immunohistochemistry (IHC)
Western Blot (WB)

Shipping:

4°C

Storage:

-20°C

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Code	Size	Price

MBL-D199-3

100 ug

£301.00

Quantity:

Prices exclude any Taxes / VAT

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Further Information

Applications:

WB - 1 ug/mL (chemiluminescence detection system) IHC - 1 ug/mL (frozen sections)

Background:

Apoptotic cells are rapidly removed by phagocytosis to clear the living cells and tissues of intracellular materials being released from dying cells. Phagocytes recognize apoptotic cells through milk fat globule-EGF-factor 8 (MFG-E8). MFG-E8 is a secreted glycoprotein from thioglycollate-elicited macrophages and bind to phosphatidylserine exposed on the cell surface of apoptotic cells, then bind strongly to cells expressing αvβ3 integrin via its RGD (arginine-glycine-aspartate) motif. Thus, MFG-E8 is an important protein to mediate the engulfment of apoptotic cells by activated macrophages.

Concentration:

1 mg/mL

Formulation:

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains nopreservatives.

Gene IDs:

Human: 4240 Mouse: 17304

Immunogen Translated:

Recombinant mouse MFG-E8

Reactivity:

This antibody reacts with mouse MFG-E8 short and long forms on Western blotting and Immunohistochemistry.

Shelf Life:

1 year

Source:

This antibody was purified from hybridoma (clone 18A2-G10) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell with Armenian hamster lymph node immunized with the recombinant mouse MFG-E8.

Target:

MFG-E8

Documents

Datasheet

References

1) Middendorp, S ., et al., Blood. 114 , 2280 - 9 (2 009) [IHC] 2) Kranich, J., et al ., J . Exp . Med . 205 , 1293 - 1302 (2008) [IH C ] 3) Aziz , M . M . et al. , Apoptosis. 13 , 609 - 20 (2008) [WB] 4) Yoshino, M . et al. , Immunol. Lett. 115 , 131 - 137 (2008) [IHC] 5) Jinushi, M ., et al ., J . Clin . Invest. 117 , 1902 - 1913 (2007) [IHC] 6) Hanayama, R., et al., PNAS 102 , 16886 - 16891 (2005) 7) Hanayama, R., et al., Science 304 , 1147 - 1150 (2004) 8) Miyasaka, K., et al., Eur. J. Immunol. 34 , 1414 - 1422 (2004) 9) Hanayama, R., et al., J. Immunol . 172 , 3876 - 3882 (2004) 10) Hanayama, R., et al., Nature 417 , 182 - 187 (2002)