Anti-CD52 (CAMPATH-1) (Mouse) mAb

IP - 20 ug/mL of cell extract from 5x106 cells FCM - 10ug/mL (final concentration)

CAMPATH-1, also known as CD52, is a heavily glycosylated, GPI-anchored protein expressed at high levels on almost all thymocytes, lymphocytes, monocytes, and macrophages. The function of CD52 is unknown. However, CD52 is an exceptionally good target for complement-mediated cell lysis and antibody-mediated cellular cytotoxicity. Humanized CAMPATH-1antibodies have been used therapeutically to effectively deplete lymphocytes in allogeneic bone marrow transplants, hematologic malignancies, and autoimmune diseases. Clinical trials suggest CAMPATH-1 antibodies are especially promising in the treatment of leukemia, non-Hodgkin lymphomas, and rheumatoid arthritis. The apparent size of CAMPATH-1 by SDS-PAGE is 25-29 kDa; however, the actual molecule is much smaller, ~ 8-9 kDa as confirmed by total structure analysis and mass spectrometry.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains nopreservatives.

Human: 1043 Mouse: 23833

Transgenic mouse spleen cell IL-2Ralpha

This antibody reacts with mouse CAMPATH-1/CD52 on Flow cytometry.

1 year

This antibody was purified from hybridoma (clone BTG-2G) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Wister rat splenocyte immunized with mouse IL-2R alpha-TGM splenocyte.

CAMPATH-1/CD52