Anti-Histone H2A mAb

WB - 1 ug/mL (chemiluminescence detection system) IP - 2 ug/200 uL of cell extract from 5x106 cells ICC - 1 ug/mL

The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes multiple different post-translational modifications including acetylation, phosphorylation, ubiquitination, methylation and ADP-ribosylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. The role of histone variants, and specially those of H3 and H2A, in various nuclear processes has been long appreciated. There are at least three different families of H2A variants present in a variety of organisms from yeast to mammals, and the degree of conservation among members of each family is greater than that of the canonical H2A. H2AX is thought to play a role in DNA double-strand break repair; the serine in the SQEY motif of H2AX is phosphorylated at the site of the DNA damage and serves as a signal for the recruitment of repair proteins. Macro H2A1, another H2A variant, has been shown to have a role in X-chromosome inactivation and dosage compensation in mammals, where it is found to localize to the inactive X after silencing has been established.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains nopreservatives.

Protein fraction was purified from mitotic chromosome

This antibody reacts with Histone H2A (14 kDa) on Western blotting, Immunoprecipitation and Immunocytochemistry.

1 year

This antibody was purified from hybridoma (clone C10037) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0-Ag14 with Balb/c mouse splenocyte immunized with the protein fraction extracted from the mitotic chromosome.

Histone H2A