MBL

Anti-Histone H2B mAb

Product Code:
 
MBL-D212-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
C14264
Regulatory Status:
 
RUO
Target Species:
  • Hamster
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
4°C
Storage:
 
-20°C
1 / 1
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MBL-D212-3100 ug£296.00
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  • Further Information
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Further Information

Applications:
WB - 1 ug/mL (chemiluminescence detection system) IP - 2 ug/200 uL of cell extract from 5x106 cells ICC - 1 ug/mL
Background:
The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes multiple different post-translational modifications including acetylation, phosphorylation, ubiquitination, methylation, and ADP-ribosylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. Acetylation of histone H2B was suppressed to greater extent than that of the others, with histone H3 being relatively least affected. The analysis of acetylation status of each of the four lysine sites at the N-terminal tail of histone H2B revealed decreases consistent with those observed in the total acetylation patterns, with the K12 and K20 residues being markedly more affected than K5 and K15 residues.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains nopreservatives.
Immunogen Translated:
Protein fraction was purified from mitotic chromosome
Reactivity:
This antibody reacts with Histone H2B (14 kDa) on Western blotting, Immunoprecipitation, and Immunocytochemistry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone C14264) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0-Ag14 with Balb/c mouse splenocyte immunized with the protein fraction extracted from the mitotic chromosome.
Target:
Histone H2B

Documents

References

1) Higashi, T., et al., J. Biotechnol. 120, 262-272 (2005) 2) Maresca, T. J., et al., J. Cell Biol. 169, 859-869 (2005) 3) Uchiyama, S., et al., J. Biol. Chem. 280, 16994-17004 (2005) 4) Vettese-Dadey, M., et al., EMBO J. 15, 2508-2518 (1996) 5) van Holde, K. E., Chromatin (Springer-Verlag, Berlin) 69-343 (1988) Clone C14264 is used in reference number 1).