MBL

Anti-CD274 (PD-L1) (Human) mAb

Product Code:
 
MBL-D230-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
27A2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Immunohistochemistry (IHC)
Shipping:
 
4°C
Storage:
 
-20°C
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2 / 2
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MBL-D230-3100 ug£301.00
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  • Further Information
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Further Information

Applications:
FCM - 10 ug/mL (final concentration) IHC - 10 ug/mL (Heat treatment required for paraffin)
Background:
Programmed death ligand 1 (PD-L1, also known as CD274/B7-H1), a member of B7 family was identified by searching for molecules that share homology with the immunogloblin V and C domains of B7-1 and B7-2 among the human cDNA expressed sequence tags in the National Center for Biotechnology Information database. PD-L1 is a ligand for programmed death 1 (PD-1) which belongs to the CD28/CTLA4 subfamily. Although in vitro study indicated that the cross-linking of PD-1 by PD-L1 leads to down-regulation of T-cell responses, some studies have shown that T cells stimulated with low levels of anti-CD3 and immobilized PD-L1-Ig were activated, proliferation and production of IFN-γ GM-CSF and IL-10 from the T cells were enhanced. The role of PD-L1 is now debatable.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
Gene IDs:
Human: 29126 Mouse: 60533
Immunogen Translated:
Recombinant human PD-L1 extracellular domain
Reactivity:
This antibody reacts with CD274 antigen on Flow cytometry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 27A2) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell SP2/0 with Balb/c mouse splenocyte immunized with recombinant human PD-L1 extracellular domain.
Target:
CD274

Documents

References

1) Droeser, R. A., et al., Eur. J. Cancer (2013) In press [IHC] 2) Gadiot, J., et al., Cancer 117, 2192-2201 (2011) [IHC] 3) Hino, R., et al., Cancer 116, 1757-1766 (2010) [IHC] 4) Schuchmann, M., et al., Am. J. Transplant. 8, 2434-2444 (2008) [IHC] 5) Hamanishi, J., et al., PNAS. 104, 3360-3365 (2007) [IHC] 6) Trautmann, L., et al., Nat. Med. 12, 1198-2202 (2006) 7) Day, C. L., et al., Nature 443, 350-354 (2006) 8) Thompson, R. H., et al., PNAS. 101, 17174-17179 (2004) This clone is used in the reference number 1)-5).