Anti-CD63 (LAMP-3) (Mouse) mAb

WB - 2-10 ug/mL (chemiluminescence detection system) FCM - 10 ug/mL (final concentration)

CD63 is not only expressed on activated platelets, but also activated monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and translocated to cell surface upon various stimuli. The membrane of lytic granules in CTLs contains CD63/LAMP-3 and other lysosomal-associated glycoproteins (LAMPs) such as CD107a/LAMP-1 and CD107b/LAMP-2. LAMPs have been observed on the cell surface as a result of degranulation. CD63 belongs to a member of the tetraspanin transmembrane-protein (TM4) superfamily, which includes CD9, CD37, CD53, CD81, CD82, CD151 and CD231. Several members of this family form noncovalent associations with integrins, particularly β1 integrins (CD29), and modulate cellular adhesion properties. CD63 has a tyrosine-based internalization motif in the cytoplasmic C-terminal tail and interacts with adaptor protein complexes such as AP-2 and AP-3. Because AP-2 and AP-3 are involved in facilitating the clathrin-mediated endocytosis, CD63 could be directly involved in the internalization of its membrane protein partners.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.

Human: 967 Mouse: 12512

Mouse bone marrow stromal cell line ST2

This antibody reacts with mouse CD63 on Western blotting and Flow cytometry.

1 year

This antibody was purified from hybridoma (clone R5G2) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Sprague-Dawley rat splenocyte immunized with mouse bone marrow stroma cell line (ST2).

CD63