MBL

Anti-Mincle (Mouse) mAb

Product Code:
 
MBL-D266-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Rat
Antibody Isotype:
 
IgG1 κ
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
1B6
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Flow Cytometry
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
4°C
Storage:
 
-20°C
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MBL-D266-3100 ul£345.00
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Further Information

Applications:
WB - 1 ug/mL (chemiluminescence detection system) IP - 2 ug/2 x 106 cells/sample FCM - 2 ug/mL Function - 1-10 ug/mL for blocking, Reference 1) and 2)
Background:
Macrophage-inducible C-type lectin (Mincle, also called as Clec4e and Clecsf9), a type ? transmembrane C-type lectin receptor, is expressed mainly in macrophages. Mincle is selectively associated with the Fc receptor common gamma-chain which contains immunoreceptor tyrosine-based activation motif (ITAM) and activates macrophages to produce inflammatory cytokines and chemokines. Mincle expressing cells are activated by spliceosome associated protein 130 (SAP130) which is a component of small nuclear ribonucleoprotein released from dead cells. Recently, Mincle is reported to recognize Mycobacterium tuberculosis as well as pathogenic fungus Malassezia species. Mincle is demonstrated to be an essential receptor for a mycobacterial glycolipid, trehalose-6,6'-dimycolate (TDM). The cytokine/chemokine production of macrophages which Malassezia or M.tuberculosis induces in Mincle-/- mice is significantly impaired. Mincle is considered to play a crucial role in immune responses to pathogens.
Concentration:
1 mg/mL
Description:
Anti-Mincle (Mouse) mAb (clone: 1B6) can be used for WB, IP, FCM and blocking. Clone 1B6 has high sensitivity in WB and IP.
Formulation:
PBS containing 50% Glycerol (pH 7.2). No preservative is contained.
Gene IDs:
Mouse: 56619
Immunogen Translated:
RBL-2H3 cells expressing full-length mouse mincle
Reactivity:
This clone reacts with mouse Mincle (Clec4e) and crossreacts weakly with MCL (Clec4d).
Shelf Life:
1 year
Source:
Purified IgG from hybridoma supernatant
Target:
Mincle

Documents

References

1) Yamasaki, S., et al., Nat. Immunol. 9, 1179-1188 (2008) [WB, FCM, Function] 2) Miyake, Y., et al., Immunity 38, 1050-1062 (2013) [FCM, Function] 3) Yamasaki, S., et al., PNAS 106, 1897-1902 (2009) 4) Ishikawa, E., et al., J. Exp. Med. 206, 2879-2888 (2009)