Anti-Np95 (Mouse) mAb

WB - 1 ug/mL (chemiluminescence detection system) FCM - Reference 7) ICC - 2 ug/mL for fluorescence detection system IHC - Reference 7) (frozen section)

DNA methylation inheritance is the process of copying pre-existing methylation patterns onto newly replicated DNA strand after DNA replication. Dnmt1 is the maintenance DNA methyltransferase, which methylates hemimethylated CpGs, appears after DNA repairs and replications. The mouse Np95, also known as Uhrf1, is a nuclear protein, which contains an ubiquitin-like domain, tandem Tudor domains, a PHD (Plant Homeodomain) finger, an SRA (SET-and RING-associated) domain, and a RING (Really Interesting New Gene) finger domain. Np95 recognizes hemimethylated CpGs via the SRA domain, and recruits Dnmt1 to the sites to transfer the methylation patterns onto the newly synthesized DNA strand. Therefore, the interaction between Np95 and Dnmt1 is necessary for the inheritance of DNA methylation.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 29128 Mouse: 18140

Mouse thymic lymphoma

This antibody reacts with endogenous mouse Np95 (95 kDa).

1 year

This antibody was purified from hybridoma (clone Th-10a) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma P3-X63-Ag8.653 cells with Wister Rat spleen cells immunized with a murine thymic lymphoma (TIGN) developed from B10.Thy1.1 mice 6 months after split-dose irradiation.

NP95