Anti-His-tag mAb
| Code | Size | Price |
|---|
| MBL-D291-3 | 200 ul | £345.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: OGHis
Regulatory Status: RUO
Applications:
- Flow Cytometry
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 0.2 ug/mL (chemiluminsence detection system) IP - 1 ug/sample FCM - 0.2 ug/mL ICC - 0.5 ug/mL
Background:
The His-tag (6xHis-tag) is one of the
most common tags used to facilitate the purification of
recombinant proteins. Metal chelate affinity
chromatography is widely used for purification of
His-tagged proteins. This specific antibody is useful tool
for monitoring of the His-tagged proteins, and recognizes
His-tags placed at N-terminal, C-terminal, and internal
regions of the recombinant proteins.
Concentration:
1 mg/mL
Formulation:
200 ug IgG in 200 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
Gene IDs:
Human: 57016 Mouse: 15109
Immunogen Translated:
6xHis-tagged protein
Reactivity:
This antibody recognizes His-tagged
protein on Western blotting, Immunoprecipitation,
Immunocytochemistry and Flow cytometry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone OGHis) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell SP-1 with Balb/c mouse splenocyte immunized with
6xHis tagged protein.
Target:
His
References
Kawada J et al. Heteroclitic serological response in esophageal and prostate cancer patients after NY-ESO-1 protein vaccination. Int J Cancer 130, 584-92 (2012) (PMID:21413013)Application: WB
Suzuki T et al. Monoubiquitination of Tob/BTG family proteins competes with degradation-targeting polyubiquitination. Biochem Biophys Res Commun. 409, 70-4 (2011) (PMID:21549103)Application: WB
Li T et al. RNF168-mediated H2A neddylation antagonizes its ubiquitination and regulates DNA damage repair. J Cell Sci. (2014) In press. (PMID:24634510)Application:
Hu B et al. HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation. Nucleic Acids Res. 42, 5582-93 (2014) (PMID:24711370)Application: WB