Anti-His-tag mAb-Agarose
| Code | Size | Price |
|---|
| MBL-D291-8 | 400 ul | £307.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: OGHis
Regulatory Status: RUO
Application: Immunoprecipitation (IP)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
IP - 20 uL/300 uL of cell extract from 1 x 106 cells
Background:
The His-tag (6xHis-tag) is one of the
most common tags used to facilitate the purification of
recombinant proteins in Escherichia coli or other
expression systems.
This product is useful tool for immunoprecipitation of the
His-tagged proteins, and it recognizes His-tags placed at
N-terminal, C-terminal, and internal regions of the
recombinant proteins.
Conjugate:
Agarose
Formulation:
200 ug of anti-His-tag monoclonalantibody covalently coupled to 200 ul of agarose gel andprovided as a 50% gel slurry suspended in PBS containingpreservative (0.09% sodium azide) for a total volume of400 ul.*Azide may react with copper or lead in plu
Immunogen Translated:
6xHis-tagged protein
Reactivity:
This antibody recognizes His-tag
peptide sequence on Immunoprecipitation.
Shelf Life:
1 year
Target:
His
References
1) Kawada, J., et al., Int. J. Cancer 130, 584-592 (2012)
2) Ueyama, T., et al., J. Biol. Chem. 286, 40693-40705 (2011)
3) Suzuki, T., et al., Biochem. Biophys. Res. Commun.409, 70-74 (2011)
4) Hiragami-Hamada, K., et al., Mol. Cell Biol.31, 1186-1200 (2011)
Clone OGHis is used in these references.