Anti-His-tag mAb-Alexa Fluor 488
| Code | Size | Price |
|---|
| MBL-D291-A48 | 50 uL | £323.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: OGHis
Regulatory Status: RUO
Applications:
- Flow Cytometry
- Immunocytochemistry (ICC)
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Applications:
FCM - 0.5 ug/mL (final concentration) ICC - 1 ug/mL
Background:
The His-tag (6xHis-tag) is one of the
most common tags used to facilitate the purification of
recombinant proteins. Metal chelate affinity
chromatography is widely used for purification of
His-tagged proteins. This specific antibody is useful tool
for monitoring of the His-tagged proteins, and recognizes
His-tags placed at N-terminal, C-terminal, and internal
regions of the recombinant proteins.
Concentration:
1 mg/mL
Formulation:
50 ug of IgG in 50 ul volume of PBS containing 1% BSA and 0.09% NaN3.*Azide may react with copper or lead in plumbing system to formexplosive metal azides. Therefore, always flush plenty of waterwhen disposing materials containing azide into drain.
Gene IDs:
Human: 3034
Immunogen Translated:
6xHis-tagged protein
Reactivity:
This antibody recognizes His-tagged
protein on Immunocytochemistry and Flow cytometry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
(clone OGHis) supernatant using protein A agarose. This
hybridoma was established by fusion of mouse myeloma
cell SP-1 with Balb/c mouse splenocyte immunized with
6xHis tagged protein.
Target:
His