Anti-Mincle (Mouse) mAb (Functional Grade)
| Code | Size | Price |
|---|
| MBL-D292-3M2 | 100 uL | £404.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG1 κ
Antibody Clonality: Monoclonal
Antibody Clone: 4A9
Regulatory Status: RUO
Target Species: Mouse
Application: Blocking
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
Function - 1-10 ug/mL for blocking, Reference 1)
Background:
Macrophage-inducible C-type lectin (Mincle, also called as Clec4e and Clecsf9), a type ?U transmembrane C-type lectin receptor, is expressed mainly in macrophages. Mincle is selectively associated with the Fc receptor common γ-chain which contains immunoreceptor tyrosine-based activation motif (ITAM) and activates macrophages to produce inflammatory cytokines and chemokines. Mincle expressing cells are activated by spliceosome associated protein 130 (SAP130) which is a component of small nuclear ribonucleoprotein released from dead cells. Recently, Mincle is reported to recognize Mycobacterium tuberculosis as well as pathogenic fungus Malassezia species. Mincle is demonstrated to be an essential receptor for a mycobacterial glycolipid, trehalose-6,6'-dimycolate (TDM). The cytokine/chemokine production of macrophages which Malassezia or M.tuberculosis induces in Mincle-/- mice is significantly impaired. Mincle is considered to play a crucial role in immune responses to pathogens.
Concentration:
1 mg/mL
Formulation:
PBS. Azide free, 0.22 um sterile-filteredEndotoxin level is < 0.5 EU/mg antibody, as determined by the LAL assay.
Gene IDs:
Mouse: 56619
Immunogen Translated:
RBL-2H3 cells expressing full-length mouse mincle
Reactivity:
This clone reacts with mouse Mincle (Clec4e).
Shelf Life:
1 year
Source:
Purified IgG from hybridoma supernatant
Target:
CLEC4E
References
1) Yamasaki, S., et al., Nat. Immunol. 9, 1179-1188 (2008)
2) Yamasaki, S., et al., PNAS 106, 1897-1902 (2009)
3) Ishikawa, E., et al., J. Exp. Med. 206, 2879-2888 (2009)