Anti-Integrin α7 (Mouse) mAb-FITC
Code | Size | Price |
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MBL-K0046-4 | 100 ul | £204.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3C12
Regulatory Status: RUO
Target Species: Mouse
Application: Flow Cytometry
Shipping:
4°C
Storage:
4°C
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Further Information
Applications:
FCM - 25-50 ug/mL (final concentration)
Background:
The integrin family of adhesion
molecules participate in important cell-cell and
cell-extracellular matrix interactions in a diverse range of
biological processes. Integrins are heterodimers consisting
of an α subunit and a β subunit. Both α and β subunits are
transmembrane proteins with large extracellular domains
(>100 kDa for α subunit and >75 kDa for β subunit) that
interact with extracellular matrix proteins and relatively
small cytoplasmic domains (50 amino acids or less, except
for the β4 subunit) that interact with cytoskeletal proteins.
The adhesiveness of integrins is dynamically regulated in
response to cytoplasmic signals, termed ?inside-out?
signaling. It has been reported that, upon ligand binding,
integrins regulate many intracellular signaling pathways
that involve cytoplasmic alkalization, intracellular Ca2+
fluctuation, inositol lipid metabolism, protein kinase C,
MAP kinase and phosphatidyl inositol kinase. Integrin α7
is a specific cellular receptor for the basement membrane
protein laminin-1, as well as for the laminin isoforms-2 and
-4. The α7 subunit is expressed mainly in skeletal and
cardiac muscle and may be involved in differentiation and
migration processes during myogenesis. Absence of
integrin α7 results in muscular dystrophy is revealed.
Concentration:
500 ug/ml
Conjugate:
FITC
Formulation:
50 ug IgG in 100 ul volume of PBS containing 1% BSA and 0.1% ProClin150.*Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush plenty ofwater when disposing materials containing azide into drain.
Gene IDs:
Human: 3679 Mouse: 16404
Immunogen Translated:
Mouse myoblast
Reactivity:
This antibody reacts with mouse Integrin
α7 on Flow cytometry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma
using protein A agarose. This hybridoma was established
by fusion of mouse myeloma cell SP2/0 with Integrin α7
knockout C57B/6 mouse splenocyte immunized with
mouse myoblasts.
References
1) Samson, T., et al., J. Biol. Chem. 282, 15730-15742 (2007)
2) Samson, T., et al., J. Biol. Chem. 279, 28641-28652 (2004)
3) Volpers, C., et al., J. Virol. 77, 2093-2104 (2003)
4) Rosbottom, A., et al., J. Immunol. 169, 5689-5695 (2002)
5) von der Mark, H., et al., J. Biol. Chem. 277, 6012-6016 (2002)
6) Mielenz. D., et al., J. Biol. Chem. 276, 13417-13426 (2001)
Clone 3C12 is used in these references.